PXD022358

PXD022358 is an original dataset announced via ProteomeXchange.

Title	Mesenchymal stem cell derived exosomal microRNA-34c-5p ameliorates renal pericyte activation by inhibiting core fucosylation
Description	Renal interstitial fibrosis(RIF) is an incurable pathological lesion in progressive chronic kidney diseases. Myofibroblasts proliferation and microvascular damage are two important events of RIF and pericyte is one of the major sources of myofibroblasts in kidney. However, the underlying mechanisms remain poorly characterized. Here we present that core fucosylation (CF), a posttranslational modification of proteins, is essential for pericyte activation via regulating of profibrotic and antifibrotic signaling pathway as a “hub-like” target. Exosomes deriving from MSCs specifically reside in obstructed kidney and deliver microRNA34c-5p to inhibit CF resulting in reducing pericyte activation and renal fibrosis. Furthermore, we clarify that the ligands and receptors (CD81-EGFR) complex assists the entrance of exosomal microRNA34c-5p into pericytes. Our results reveal a novel mechanism of pericyte activation base on CF and suggest a potential use of MSCs exosome as a new therapeutic strategy for renal interstitial fibrosis through inhibiting CF, the “hub-like” target of profibrotic signaling activation.
HostingRepository	iProX
AnnounceDate	2020-11-04
AnnouncementXML	Submission_2021-11-23_19:40:57.759.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuemei Hu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-04 15:05:12	ID requested
⏵ 1	2021-11-23 19:40:58	announced

Hu X, Shen N, Liu A, Wang W, Zhang L, Sui Z, Tang Q, Du X, Yang N, Ying W, Qin B, Li Z, Li L, Wang N, Lin H, Bone marrow mesenchymal stem cell-derived exosomal miR-34c-5p ameliorates RIF by inhibiting the core fucosylation of multiple proteins. Mol Ther, 30(2):763-781(2022) [pubmed]

submitter keyword: mesenchymal stem cells, exosomes, pericyte, core fucosylation, renal interstitial fibrosis, microRNA-34c-5p

Hongli Lin
contact affiliation	The First Affiliated Hospital of Dalian Medical University
contact email	linhongli@163.com
lab head
Xuemei Hu
contact affiliation	The First Affiliated Hospital of Dalian Medical University
contact email	18663827106@163.com
dataset submitter

iProX dataset URI