PXD022354

PXD022354 is an original dataset announced via ProteomeXchange.

Title	Epithelial cell line derived from endometriotic lesions mimics macrophage nervous mechanism of pain generation on proteome and metabolome levels
Description	Endometriosis is a benign disease affecting one in ten women of reproductive age worldwide. Although the pain level is not correlated to the extent of the disease it still is one of the cardinal symptoms strongly affecting the patients’ quality of life. Yet, a molecular mechanism leading to the formation of severe pain remains to be defined. Recent studies have indicated a close interaction between newly generated nerve cells and macrophages, leading to neurogenic inflammation in the pelvic area. In this context, the responsiveness of an endometriotic cell culture model was characterized upon inflammatory stimulation by employing a multi-omics approach, including proteomics and metabolomics. Differential proteomic profiling of the 12-Z endometriotic cell line treated with TNFα and IL1β unexpectedly showed that the inflammatory stimulation was able to induce a protein signature associated with neuroangiogenesis, specifically including neuropilins (NRP1/2). Untargeted metabolomic profiling in the same setup further revealed that the endometriotic cells were capable of the autonomous production of 7,8-dihydrobiopterin (BH2), 7,8-dihydroneopterin and epinephrine. These metabolites are related to the development of neuropathic pain and the former two were even up-regulated upon the inflammatory stimulation. Thus, the inflammatory stimulation of endometriotic 12-Z cells led to protein and metabolite expression changes that strongly suggest a direct involvement of epithelial cells in endometriosis pain development.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_13:16:41.999.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022354
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-04 07:29:50	ID requested
⏵ 1	2021-09-09 13:16:42	announced

Neuditschko B, Leibetseder M, Brunmair J, Hagn G, Skos L, Gerner MC, Meier-Menches SM, Yotova I, Gerner C, Epithelial Cell Line Derived from Endometriotic Lesion Mimics Macrophage Nervous Mechanism of Pain Generation on Proteome and Metabolome Levels. Biomolecules, 11(8):(2021) [pubmed]

submitter keyword: Endometriosis, inflammation, metabolomics, multi-omics, proteomics

Christopher Gerner
contact affiliation	Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Waehringer Straße 38, 1090, Vienna, Austria
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022354
     1. Label: PRIDE project
     2. Name: Epithelial cell line derived from endometriotic lesions mimics macrophage nervous mechanism of pain generation on proteome and metabolome levels