PXD022350

PXD022350 is an original dataset announced via ProteomeXchange.

Title	Surface AMP Deaminase 2 as a Novel Regulator Modifying Extracellular Adenine Nucleotide Metabolism
Description	Adenine nucleotides represent crucial immunomodulators in the extracellular environment. The ectonucleotidases CD39 and CD73 are responsible for the sequential catabolism of ATP to adenosine via AMP, thereby promoting an anti-inflammatory milieu induced by the “adenosine halo”. AMPD2 intracellularly mediates AMP deamination to IMP, thus constituting an ambiguous mediator both enhancing the degradation of inflammatory ATP and reducing the formation of protective adenosine. Here, we show that this enzyme is expressed on the cell surface of human immune cells and its predominance may[BF1] modify inflammatory states by altering the extracellular milieu. Surface AMPD2 (eAMPD2) expression on monocytes was verified by immunoblot, mass spectrometry, surface biotinylation, and immunofluorescence microscopy. Flow cytometry revealed enhanced monocytic eAMPD2 expression after TLR stimulation. PBMCs from patients with rheumatoid arthritis displayed significantly higher levels of eAMPD2 expression compared to healthy controls. Furthermore, we observed that extracellular adenosine production was not impaired by an enhanced eAMPD2 expression, while IMP exerted anti-inflammatory effects. In summary, our study identifies eAMPD2 as a novel regulator of the extracellular ATP-adenosine balance adding to the immunomodulatory CD39-CD73 system.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_02:38:51.494.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marieluise Kirchner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF; Q Exactive; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-04 07:29:14	ID requested
⏵ 1	2021-11-03 02:38:52	announced

Ehlers L, Kuppe A, Damerau A, Wilantri S, Kirchner M, Mertins P, Strehl C, Buttgereit F, Gaber T, Surface AMP deaminase 2 as a novel regulator modifying extracellular adenine nucleotide metabolism. FASEB J, 35(7):e21684(2021) [pubmed]

submitter keyword: Human, Epithelial Cells, Monocytes

Philipp Mertins
contact affiliation	Berlin Insitute of Health, Proteomics Platform, Charité University Medicine, Berlin, Germany
contact email	philipp.mertins@mdc-berlin.de
lab head
Marieluise Kirchner
contact affiliation	Max Delbrueck Center, Berlin
contact email	marieluise.kirchner@mdc-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022350
     1. Label: PRIDE project
     2. Name: Surface AMP Deaminase 2 as a Novel Regulator Modifying Extracellular Adenine Nucleotide Metabolism