PXD022347

PXD022347 is an original dataset announced via ProteomeXchange.

Title	Targeted deglycosylation with a nanobody-directed split OGA in living cells
Description	O-GlcNAc is an essential and dynamic post-translational modification present on thousands of nucleocytoplasmic proteins. Interrogating the role of O-GlcNAc on a target protein in cells is critical yet challenging without disturbing O-GlcNAc globally or after extensive glycosite mapping. Herein, we developed a nanobody-fused split O-GlcNAcase (OGA) as a targeted O-GlcNAc eraser for protein-selective O-GlcNAc removal in cells. Through systematic screening, we identified the essential domains of OGA and afforded a split OGA with limited activity, which selectively reinstated deglycosylation activity on the target protein upon fusion of a nanobody in living cells. We demonstrate the generality of the O-GlcNAc eraser against a series of target proteins and reveal that O-GlcNAc stabilizes the transcription factor c-Jun and promotes its interaction with c-Fos. Thus, nanobody-directed split OGA speeds the selective removal and functional evaluation of O-GlcNAc on individual proteins via an approach that is generalizable to a broader array of post-translational modifications.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_09:44:46.092.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022347
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christina Woo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-04 07:19:50	ID requested
⏵ 1	2022-02-16 09:44:46	announced

Ge Y, Ramirez DH, Yang B, D'Souza AK, Aonbangkhen C, Wong S, Woo CM, Target protein deglycosylation in living cells by a nanobody-fused split O-GlcNAcase. Nat Chem Biol, 17(5):593-600(2021) [pubmed]

submitter keyword: OGA

Christina Woo
contact affiliation	DEPARTMENT of CHEMISTRY & CHEMICAL BIOLOGY HARVARD UNIVERSITY
contact email	cwoo@chemistry.harvard.edu
lab head
Christina Woo
contact affiliation	Harvard University
contact email	cwoo@chemistry.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022347
     1. Label: PRIDE project
     2. Name: Targeted deglycosylation with a nanobody-directed split OGA in living cells