PXD022341

PXD022341 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Leveraging Parameter Dependencies in High-Field Asymmetric Waveform Ion-Mobility Spectrometry and Size-exclusion Chromatography for Proteome-wide Crosslinking Mass Spectrometry_protein ID dataset
Description	Ion mobility spectrometry shows great promise to tackle analytically challenging research questions by adding another separation dimension to liquid chromatography-mass spectrometry. The understanding of how analyte properties influence ion mobility has increased through recent studies but no clear rationale for the design of customized experimental settings has emerged. Here, we leverage machine learning to deepen our understanding of field asymmetric-waveform ion-mobility spectrometry (FAIMS) for the analysis of crosslinked peptides. Knowing that predominantly m/z, then size and charge state of an analyte influences the separation, we found ideal compensation voltages correlating with size-exclusion chromatography (SEC) fraction number. The effect of this relationship on analytical depth can be substantial as exploiting it allowed us to almost double unique residue pair detections in a proteome-wide crosslinking experiment. Other applications involving liquid- and gas-phase separation may also benefit from considering such parameter dependencies.
HostingRepository	jPOST
AnnounceDate	2022-12-16
AnnouncementXML	Submission_2024-05-02_05:01:22.012.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ludwig Sinn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	alpha-amino acetylated residue; L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; S-carboxamidoethyl-L-cysteine
Instrument	instrument; Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-04 02:01:48	ID requested
1	2022-12-15 07:00:30	announced
⏵ 2	2024-05-02 05:01:24	announced	2024-05-02: Updated PubMed.

Publication List

Sinn LR, Giese SH, Stuiver M, Rappsilber J, Leveraging Parameter Dependencies in High-Field Asymmetric Waveform Ion-Mobility Spectrometry and Size Exclusion Chromatography for Proteome-wide Cross-Linking Mass Spectrometry. Anal Chem, 94(11):4627-4634(2022) [pubmed]

Sinn LR, Giese SH, Stuiver M, Rappsilber J, Leveraging Parameter Dependencies in High-Field Asymmetric Waveform Ion-Mobility Spectrometry and Size Exclusion Chromatography for Proteome-wide Cross-Linking Mass Spectrometry. Anal Chem, 94(11):4627-4634(2022) [pubmed]

Keyword List

submitter keyword: FAIMS, chromatography, SEC, CLMS, PPI

submitter keyword: FAIMS, chromatography, SEC, CLMS, PPI

Contact List

Prof. Dr. Juri Rappsilber
lab head
Ludwig Sinn
contact affiliation	Technische Universität Berlin, Bioanalytics, Prof. Juri Rappsilber
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000990/