PXD022334

PXD022334 is an original dataset announced via ProteomeXchange.

Title	Proteomic and phosphoproteomic profiling of SFTPCI73T expressing patient-specific pluripotent stem cell-derived alveolar epithelial type 2 cells and their gene-edited corrected counterparts.
Description	We performed proteomic and phosphoproteomic profiling of cells derived from human induced pluripotent stem cells (iPSCs) using our previously described distal lung directed differentiation protocol to generate alveolar epithelial type 2 cells (iAEC2s). We used the SPC2 human iPSC line and specifically the SPC2-ST-B2 (SFTPCtdT/WT) and SPC2-ST-C11 (SFTPCI73T/tdT) clones containing a SFTPCtdTomato knock-in reporter. SFTPCtdTomato+ cells were sorted on day 41 and again on day 79 of differentiation. iAEC2s were single-cell passaged in self-renewing 3D alveolosphere cultures approximately every 2 weeks through day 113. Live SFTPCtdTomato+ iAEC2s were sorted on day 113 and processed for mass spectrometry. We find that mutant (SFTPCI73T/tdT) iAEC2s display a less proliferative and more mature AEC2 phenotype compared to their corrected (SFTPCtdT/WT) counterparts with a concomitant upregulation of lysosomal and autophagy related pathways and activation of the NF-κB pathway in mutant cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:40.895.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ryan Hekman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-04 01:38:30	ID requested
1	2021-10-07 08:48:21	announced
⏵ 2	2023-11-14 08:51:41	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: autophagy, proteomics,pluripotent stem cells, pulmonary fibrosis, alveolar epithelial type 2 cells

Darrell N. Kotton
contact affiliation	Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA The Pulmonary Center and Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
contact email	dkotton@bu.edu
lab head
Ryan Hekman
contact affiliation	Boston University
contact email	rhekman@bu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022334
     1. Label: PRIDE project
     2. Name: Proteomic and phosphoproteomic profiling of SFTPCI73T expressing patient-specific pluripotent stem cell-derived alveolar epithelial type 2 cells and their gene-edited corrected counterparts.