PXD022328

PXD022328 is an original dataset announced via ProteomeXchange.

Title	Virulence factors associated with amoebic gill disease from Neoparamoeba perurans
Description	The parasitic amoeba, Neoparamoeba perurans is the causative agent of Amoebic Gill Disease in salmonids. The parasite has previously been reported to lose virulence during prolonged in vitro maintenance. In this study, the impact of prolonged culture on N. perurans virulence and its proteome was investigated. Three isolates of N. perurans maintained in culture for varying durations were compared. Two isolates, attenuated and virulent, had their virulence assessed in an experimental trial using Atlantic salmon smolts and their bacterial community composition was evaluated by 16S rRNA Illumina MiSeq sequencing. Soluble proteins were isolated from a newly acquired, virulent and attenuated N. perurans culture and were analysed using two-dimensional electrophoresis (2D PAGE) coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS). An experimental challenge trial using Atlantic salmon smolts confirmed a loss in virulence in an N. perurans culture that was maintained in vitro for 3 years. A greater diversity of bacterial communities was found in the microbiome of the virulent isolate harbouring predominant genera belonging to Pseudoaltermonas spp, Vibrio spp and Fluviicola spp. Microbial community richness was reduced in the attenuated microbiome, with a singular species, Thalassopira xiamenensis, representing a large proportion of its microbiome. A collated proteome database of N. perurans, Amoebozoa and four bacterial genera resulted in 24 proteins differentially expressed between the three cultures. The present LC-MS/MS results indicate protein synthesis, oxidative stress and the plausible occurrence of immunomodulation are ultimately upregulated in a newly acquired N. perurans culture and future studies may exploit these protein identifications for therapeutic purposes in infected farmed fish.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_09:43:08.942.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Orla Slattery
SpeciesList	scientific name: Paramoeba perurans; NCBI TaxID: 437603;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-04 01:37:50	ID requested
⏵ 1	2022-02-16 09:43:10	announced

Dhufaigh KN, Dillon E, Botwright N, Birlanga V, Talbot A, O'Connor I, MacCarthy E, Slattery O, Comparative proteomic profiling of newly acquired, virulent and attenuated Neoparamoeba perurans proteins associated with amoebic gill disease. Sci Rep, 11(1):6830(2021) [pubmed]

submitter keyword: Amoebic gill disease, Virulence factors, Neoparamoeba perurans, Microbiome, LC- MS/MS

Orla Slattery
contact affiliation	Department of Biopharmaceutical and Medical Science, Galway-Mayo Institute of Technology
contact email	orla.slattery@gmit.ie
lab head
Orla Slattery
contact affiliation	GMIT
contact email	orla.slattery@gmit.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022328
     1. Label: PRIDE project
     2. Name: Virulence factors associated with amoebic gill disease from Neoparamoeba perurans