PXD022325 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis to examine the functional consequences of intratumor heterogeneity |
Description | To examine the functional consequences of intratumor heterogeneity, subclonal populations (SCPs) were derived from the MDA-MB-468 triple-negative breast cancer cell line. Characterization of these SCPs revealed considerable variation in SCP tumor forming capacity with SCP #32 (SCP32) having an exceptional high tumor forming capacity. To investigate the proteomic differences between tumors of SCP32 and other SCPs, in TMT1, isobaric tandem mass tag (TMT) labeling combined with LC-MS/MS (TMT-MS) was performed on tumors of SCP03, SCP29 and SCP32 (n = 3 each) collected at 2 months post transplantation. In TMT2, isobaric tandem mass tag (TMT) labeling combined with LC-MS/MS (TMT-MS) was performed on tumors of the parental MDA-MB-468 cell line (n = 2), and both barcoded and non-barcoded versions SCP29 and SCP32 (two each) collected at 2 months post transplantation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:32:18.836.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tian Zhang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-04 01:37:34 | ID requested | |
1 | 2022-02-16 09:42:53 | announced | |
⏵ 2 | 2024-10-22 05:32:26 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1038/s41388-021-02075-y; |
Kuiken HJ, Dhakal S, Selfors LM, Friend CM, Zhang T, Callari M, Schackmann RCJ, Gray GK, Crowdis J, Bhang HC, Baslan T, Stegmeier F, Gygi SP, Caldas C, Brugge JS, Clonal populations of a human TNBC model display significant functional heterogeneity and divergent growth dynamics in distinct contexts. Oncogene, 41(1):112-124(2022) [pubmed] |
Keyword List
submitter keyword: Subclonal population (SCPs), Intratumor heterogeneityr, Human breast cancer cell line,TMT, Proteomics,Mass spectrometry, MDA-MB-468 |
Contact List
Steven Gygi |
contact affiliation | Harvard Medical School |
contact email | steven_gygi@hms.harvard.edu |
lab head | |
Tian Zhang |
contact affiliation | Harvard Medical School |
contact email | tian_zhang@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD022325 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022325
- Label: PRIDE project
- Name: Proteomic analysis to examine the functional consequences of intratumor heterogeneity