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PXD022325

PXD022325 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis to examine the functional consequences of intratumor heterogeneity
DescriptionTo examine the functional consequences of intratumor heterogeneity, subclonal populations (SCPs) were derived from the MDA-MB-468 triple-negative breast cancer cell line. Characterization of these SCPs revealed considerable variation in SCP tumor forming capacity with SCP #32 (SCP32) having an exceptional high tumor forming capacity. To investigate the proteomic differences between tumors of SCP32 and other SCPs, in TMT1, isobaric tandem mass tag (TMT) labeling combined with LC-MS/MS (TMT-MS) was performed on tumors of SCP03, SCP29 and SCP32 (n = 3 each) collected at 2 months post transplantation. In TMT2, isobaric tandem mass tag (TMT) labeling combined with LC-MS/MS (TMT-MS) was performed on tumors of the parental MDA-MB-468 cell line (n = 2), and both barcoded and non-barcoded versions SCP29 and SCP32 (two each) collected at 2 months post transplantation.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:32:18.836.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTian Zhang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-04 01:37:34ID requested
12022-02-16 09:42:53announced
22024-10-22 05:32:26announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41388-021-02075-y;
Kuiken HJ, Dhakal S, Selfors LM, Friend CM, Zhang T, Callari M, Schackmann RCJ, Gray GK, Crowdis J, Bhang HC, Baslan T, Stegmeier F, Gygi SP, Caldas C, Brugge JS, Clonal populations of a human TNBC model display significant functional heterogeneity and divergent growth dynamics in distinct contexts. Oncogene, 41(1):112-124(2022) [pubmed]
Keyword List
submitter keyword: Subclonal population (SCPs), Intratumor heterogeneityr, Human breast cancer cell line,TMT, Proteomics,Mass spectrometry, MDA-MB-468
Contact List
Steven Gygi
contact affiliationHarvard Medical School
contact emailsteven_gygi@hms.harvard.edu
lab head
Tian Zhang
contact affiliationHarvard Medical School
contact emailtian_zhang@hms.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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