⮝ Full datasets listing

PXD022294

PXD022294 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHakai is required for stabilization of core components of the m6A mRNA methylation machinery
DescriptionN6-methyladenosine (m6A) is the most abundant internal modification on mRNA which influences most steps of mRNA metabolism and is involved in several biological functions. The E3 ubiquitin ligase Hakai was previously found in complex with components of the m6A methylation machinery in plants and mammalian cells but its precise function remained to be investigated. Here we show that Hakai is a conserved component of the methyltransferase complex in Drosophila and human cells. In Drosophila, its depletion results in reduced m6A levels and altered m6A-dependent functions including sex determination. We show that its ubiquitination domain is required for dimerisation and interaction with other members of the m6A machinery, while its catalytic activity is dispensable. Finally, we demonstrate that the loss of Hakai destabilizes several subunits of the methyltransferase complex, resulting in impaired m6A deposition. Our work adds new functional and molecular insights into the mechanism of the m6A mRNA writer complex.
HostingRepositoryPRIDE
AnnounceDate2021-04-29
AnnouncementXMLSubmission_2021-07-19_22:54:27.662.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPetra Beli
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListubiquitinylated lysine
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-02 03:03:42ID requested
12021-04-29 03:18:07announced
22021-07-19 22:54:28announced2021-07-20: Updated publication reference for PubMed record(s): 34145251.
Publication List
Bawankar P, Lence T, Paolantoni C, Haussmann IU, Kazlauskiene M, Jacob D, Heidelberger JB, Richter FM, Nallasivan MP, Morin V, Kreim N, Beli P, Helm M, Jinek M, Soller M, Roignant JY, A mRNA methylation machinery. Nat Commun, 12(1):3778(2021) [pubmed]
Keyword List
submitter keyword: Hakai, m6A, RNA modification, sex determination
Contact List
Petra Beli
contact affiliationInstitute of Molecular Biology (IMB), Mainz, Germany
contact emailp.beli@imb-mainz.de
lab head
Petra Beli
contact affiliationInstitute of Molecular Biology (IMB), Mainz, Germany
contact emailp.beli@imb-mainz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD022294
PRIDE project URI
Repository Record List
[ + ]