PXD022294 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hakai is required for stabilization of core components of the m6A mRNA methylation machinery |
Description | N6-methyladenosine (m6A) is the most abundant internal modification on mRNA which influences most steps of mRNA metabolism and is involved in several biological functions. The E3 ubiquitin ligase Hakai was previously found in complex with components of the m6A methylation machinery in plants and mammalian cells but its precise function remained to be investigated. Here we show that Hakai is a conserved component of the methyltransferase complex in Drosophila and human cells. In Drosophila, its depletion results in reduced m6A levels and altered m6A-dependent functions including sex determination. We show that its ubiquitination domain is required for dimerisation and interaction with other members of the m6A machinery, while its catalytic activity is dispensable. Finally, we demonstrate that the loss of Hakai destabilizes several subunits of the methyltransferase complex, resulting in impaired m6A deposition. Our work adds new functional and molecular insights into the mechanism of the m6A mRNA writer complex. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-29 |
AnnouncementXML | Submission_2021-07-19_22:54:27.662.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Petra Beli |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | ubiquitinylated lysine |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-02 03:03:42 | ID requested | |
1 | 2021-04-29 03:18:07 | announced | |
⏵ 2 | 2021-07-19 22:54:28 | announced | 2021-07-20: Updated publication reference for PubMed record(s): 34145251. |
Publication List
Bawankar P, Lence T, Paolantoni C, Haussmann IU, Kazlauskiene M, Jacob D, Heidelberger JB, Richter FM, Nallasivan MP, Morin V, Kreim N, Beli P, Helm M, Jinek M, Soller M, Roignant JY, A mRNA methylation machinery. Nat Commun, 12(1):3778(2021) [pubmed] |
Keyword List
submitter keyword: Hakai, m6A, RNA modification, sex determination |
Contact List
Petra Beli |
contact affiliation | Institute of Molecular Biology (IMB), Mainz, Germany |
contact email | p.beli@imb-mainz.de |
lab head | |
Petra Beli |
contact affiliation | Institute of Molecular Biology (IMB), Mainz, Germany |
contact email | p.beli@imb-mainz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022294
- Label: PRIDE project
- Name: Hakai is required for stabilization of core components of the m6A mRNA methylation machinery