PXD022292

PXD022292 is an original dataset announced via ProteomeXchange.

Title	Primary angle closure glaucoma is characterized by altered extracellular matrix homeostasis in the iris
Description	Purpose. To characterize the proteome of the iris in primary angle closure glaucoma (PACG). Experimental Design. In this cross-sectional study, iris samples were obtained from surgical iridectomy of 48 adults with PACG and five normal controls. Peptides from iris were analyzed using liquid chromatography-tandem mass spectrometry on an Orbitrap Q Exactive Plus mass spectrometer. Verification of proteins of interest was conducted using selected reaction monitoring on a triple quadrupole mass spectrometer. The main outcome was proteins with a log2 two-fold difference in expression in iris between PACG and controls. Results. There were 3,446 non-redundant proteins identified in human iris, of which 165 proteins were upregulated and 251 proteins were downregulated in PACG compared with controls. Thirty-two upregulated proteins were either components of the extracellular matrix (ECM) (fibrillar collagens, EMILIN-2, fibrinogen, fibronectin, matrilin-2), matricellular proteins (thrombospondin-1), proteins involved in cell-matrix interactions (integrins, laminin, histidine-rich glycoprotein, paxillin), or protease inhibitors known to modulate ECM turnover (-2 macroglobulin, tissue factor pathway inhibitor 2, papilin). Two giant proteins, titin and obscurin, were up- and down-regulated, respectively, in the iris in PACG compared with controls. Conclusions and Clinical Relevance. This proteomic study shows that ECM composition and homeostasis is altered in the iris in PACG.
HostingRepository	PRIDE
AnnounceDate	2022-03-01
AnnouncementXML	Submission_2022-03-01_12:37:54.869.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022292
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pingbo Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-02 03:03:38	ID requested
⏵ 1	2022-03-01 12:37:56	announced

Semba RD, Zhang P, Dufresne C, Gao T, Al-Jadaan I, Craven ER, Qian J, Edward DP, Mahale A, Primary angle closure glaucoma is characterized by altered extracellular matrix homeostasis in the iris. Proteomics Clin Appl, 15(6):e2000094(2021) [pubmed]

ProteomeXchange project tag: EyeOME (B/D-HPP)

submitter keyword: extracellular matrix, eye, iris, primary angle closure glaucoma, proteomics

Richard D. Semba, M.D.
contact affiliation	Wilmer Eye Institute, Smith Building, M015, 400 N Broadway, Baltimore, MD 21287
contact email	rdsemba@jhmi.edu
lab head
Pingbo Zhang
contact affiliation	Johns Hopkins University
contact email	pzhang7@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022292
     1. Label: PRIDE project
     2. Name: Primary angle closure glaucoma is characterized by altered extracellular matrix homeostasis in the iris