PXD022284

PXD022284 is an original dataset announced via ProteomeXchange.

Title	Formation and characterization of biofilms formed by salt tolerant yeast strains in seawater-based growth medium
Description	Yeast whole cells have been widely used in modern biotechnology as biocatalysts to generate numerous compounds of industrial, chemical, and pharmaceutical importance. Since many of the biocatalysis utilizing manufactures have become more concerned about environmental issues, seawater is now considered a sustainable alternative to freshwater for biocatalytic processes. This approach plausibly commenced new research initiatives into exploration of salt tolerant yeast strains. Recently, there has also been a growing interest in possible applications of microbial biofilms in the field of biocatalysis. In these complex communities, cells demonstrate higher resistance to adverse environmental conditions due to their embedment in an extracellular matrix, in which physical, chemical, and physiological gradients exist. Considering these two topics, seawater and biofilms, in this work we characterized biofilm formation in seawater-based growth media by several salt tolerant yeast strains with previously demonstrated bocatalytic capacities. The tested strains formed both air-liquid-like biofilms and biofilms on silicone surfaces, with Debaryomyces fabryi, Schwanniomyces etchellsii, S. polymorphus and Kluyveromyces marxianus showing the highest biofilm formation. The extracted biofilm extracellular matrices mostly consisted of carbohydrates and proteins. The latter group was primarily represented by enzymes involved in metabolic processes, particularly the biosynthetic ones, and in the response to stimuli. Specific features were also found in the carbohydrate composition of the extracellular matrix, which were dependent both on the yeast isolate and the nature of formed biofilms. Overall, our findings presented herein provide a unique data resource for further development and optimization of biocatalytic processes and applications employing seawater and halotolerant yeast biofilms.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_13:58:21.768.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022284
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Greg Sabat
SpeciesList	scientific name: Debaryomyces hansenii var. hansenii; NCBI TaxID: 58641; scientific name: Kluyveromyces marxianus; NCBI TaxID: 4911; scientific name: Debaryomyces fabryi; NCBI TaxID: 58627;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-02 01:41:41	ID requested
⏵ 1	2021-09-09 13:58:22	announced

Zarnowski R, Sanchez H, Andreu C, Andes D, Del Olmo ML, Formation and characterization of biofilms formed by salt-tolerant yeast strains in seawater-based growth medium. Appl Microbiol Biotechnol, 105(6):2411-2426(2021) [pubmed]

submitter keyword: biofilms, extracellular matrix composition, seawater, Debaryomyces, Schwanniomyces, Kluyveromyces

Dr. Marcelli del Olmo
contact affiliation	Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, Burjassot (València), Spain
contact email	M.Del.Olmo@uv.es
lab head
Greg Sabat
contact affiliation	University of Wisconsin
contact email	gsabat@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022284
     1. Label: PRIDE project
     2. Name: Formation and characterization of biofilms formed by salt tolerant yeast strains in seawater-based growth medium