PXD022279

PXD022279 is an original dataset announced via ProteomeXchange.

Title	Multiplexed CuAAC-Suzuki-Miyaura for tandem activity-based chemoproteomic profiling
Description	Suzuki-Miyauara cross-coupling has been used for mass-spectrometry-based chemoproteomics, including for target deconvolution and site of labeling studies. We also demonstrated that Suzuki-Miyauara cross coupling and CuAAC can be combined for dual target labeling for mass spectrometry-based proteomics. Furthermore, multiplexed CuAAC Suzuki-Miyaura chemoproteomics platform has been successfully applied to identify the protein targets of bifunctional cysteine- and lysine-reactive crosslinking probes.
HostingRepository	PRIDE
AnnounceDate	2021-02-03
AnnouncementXML	Submission_2021-02-03_14:58:26.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022279
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Keriann Backus
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-01 23:45:23	ID requested
⏵ 1	2021-02-03 14:58:27	announced

Cao J, Boatner LM, Desai HS, Burton NR, Armenta E, Chan NJ, Castell, ó, n JO, Backus KM, Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem, 93(4):2610-2618(2021) [pubmed]

submitter keyword: Suzuki-Miyauara cross-coupling, multiplexed CuAAC Suzuki-Miyaura chemoproteomics , SP3 cleanup

Keriann Backus
contact affiliation	Department of Biological Chemistry, David Geffen School of Medicine, UCLA, USA
contact email	kbackus@mednet.ucla.edu
lab head
Keriann Backus
contact affiliation	UCLA
contact email	kbackus@mednet.ucla.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022279
     1. Label: PRIDE project
     2. Name: Multiplexed CuAAC-Suzuki-Miyaura for tandem activity-based chemoproteomic profiling