PXD022277

PXD022277 is an original dataset announced via ProteomeXchange.

Title	Viral lncRNA upregulates multiple A+U-richViral lncRNA upregulates multiple A+U-rich transcripts to promote cell motility and viral dissemination transcripts to promote cell motility and viral dissemination
Description	Long non-coding RNAs are frequently associated with broad modulation of gene expression and thus provide the cell with the ability to synchronize entire metabolic processes. We used transcriptomic and proteomic approaches to investigate whether the most abundant human cytomegalovirus-encoded lncRNA, RNA2.7, has this characteristic. By comparing RNA2.7 deletion mutants with wild-type virus, RNA2.7 was found to regulate a large number of cellular genes late in infection. Pathway analysis indicated that >100 RNA2.7-regulated cellular genes are associated with promoting cell movement, and ten of the most highly regulated were verified in further experiments. Morphological analysis and live cell tracking showed that RNA2.7 promotes the movement and detachment of infected cells late in infection, and plaque assays using sparse cell monolayers demonstrated that RNA2.7 promotes cell-to-cell dissemination and spread of virus. Bioinformatic analysis indicated that RNA2.7-upregulated mRNAs are relatively A+U-rich, which is a trait associated with transcript instability, and that they are also enriched in motifs associated with mRNA instability. Experiments involving transcriptional inhibition showed that transcripts from four RNA2.7-regulated cellular genes were longer-lived in the presence of RNA2.7. These findings demonstrate that RNA2.7 promotes cell movement and viral dissemination late in infection and indicate that this may be due to general stabilization of A+U-rich transcripts.
HostingRepository	PRIDE
AnnounceDate	2021-07-27
AnnouncementXML	Submission_2021-07-27_06:03:36.967.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katie Nightingale
SpeciesList	scientific name: Human herpesvirus 5 strain Merlin; NCBI TaxID: 295027; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-01 23:18:54	ID requested
⏵ 1	2021-07-27 06:03:37	announced

Dataset with its publication pending

submitter keyword: human cytomegalovirus, lncRNA, gene regulation, transcriptomics, cell motility, A+U rich, viral dissemination

Andrew Davison
contact affiliation	MRC-University of Glasgow Centre for Virus Research, Sir Michael Stoker Building, Garscube Campus, 464 Bearsden Road, Glasgow G61 1QH, Scotland (UK)
contact email	andrew.davison@glasgow.ac.uk
lab head
Katie Nightingale
contact affiliation	Cambridge Institute for Medical Research, University of Cambridge
contact email	kln25@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022277
     1. Label: PRIDE project
     2. Name: Viral lncRNA upregulates multiple A+U-richViral lncRNA upregulates multiple A+U-rich transcripts to promote cell motility and viral dissemination transcripts to promote cell motility and viral dissemination