PXD022273

PXD022273 is an original dataset announced via ProteomeXchange.

Title	Aberrant metabolism is associated with fast antibody fading in COVID-19 convalescent patients
Description	Along with the increase in the number of COVID-19 patients being recovered from the disease, it is urgently needed for better understanding the recovery processes associated with convalescent patients, particularly antibody-faded ones, to prepare for the incoming wave of COVID-19 aftermath. Herein, we conducted targeted metabolomics and lipidomics to profile plasma metabolite alterations associated with ordinary convalescent patients with antibody (CA), antibody-faded convalescent patients (CO) and healthy subjects. Besides, we also included a special group of patients with prolonged SARS-CoV-2 infection but being recovered from COVID-19 symptoms (PI). We found that the CA individuals were not fully recovered in the aspect of metabolism, and the PI individuals were marked by elevated lipid droplet biogenesis and reduced fatty acid ω-oxidation. Importantly, we identified that pentose phosphate pathway (PPP) and the levels of arachidonic acid (AA)-derived eicosanoids were aberrant in CO individuals, which were further validated via absolute quantification of oxidized lipids. Moreover, some of eicosanoids were further evaluated via ELISA using the plasma samples from an additional larger cohort and the role of certain eicosanoids in immune response related to antibody production or maintenance was experimentally exploited. Our findings show that aberrant metabolism of PPP and eicosanoids is associated with and probably contribute to the faster fading of SARS-CoV-2-specific antibodies in the CO individuals. This work provides the knowledge for better understanding host response associated with COVID-19 convalescence, shed light on the metabolic features of faster fading of SARS-CoV-2 antibodies, and provides valuable hints of therapeutic and/or vaccination strategies.
HostingRepository	iProX
AnnounceDate	2020-10-31
AnnouncementXML	Submission_2020-10-30_19:45:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Qiu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	QTRAP 6500+

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-30 19:06:29	ID requested
⏵ 1	2020-10-30 19:45:13	announced

Dataset with its publication pending

submitter keyword: COVID-19, convalescent, metabolites, antibody fading

Yang Qiu
contact affiliation	Joint Laboratory of Infectious Diseases and Health, Wuhan Institute of Virology & Wuhan Jinyintan Hospital, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences (CAS), Wuhan, China
contact email	yangqiu@wh.iov.cn
lab head
Yang Qiu
contact affiliation	Joint Laboratory of Infectious Diseases and Health, Wuhan Institute of Virology & Wuhan Jinyintan Hospital, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences (CAS), Wuhan, China
contact email	yangqiu@wh.iov.cn
dataset submitter

iProX dataset URI