PXD022270

PXD022270 is an original dataset announced via ProteomeXchange.

Title	Synaptosome proteomics of tetanus neurotoxin-injected mice
Description	Network hyperexcitability is manifested as frequent ictal discharges, often caused by unbalanced neurotransmission. We assessed synaptic changes in the hyperexcitable visual cortex of tetanus neurotoxin-injected mice (TeNT). A proteomics analysis of synaptic content revealed Carboxypeptidase E (CPE) up-regulation following TeNT injection. To quantify CPE effect on vesicle clustering, we used an ultrastructural measure of synaptic activity to investigate functional differences at excitatory and inhibitory synapses. We found homeostatic changes in hyperexcitable networks expressed as an early onset lengthening of active zones at inhibitory synapses followed by spatial reorganization at excitatory synapses. Moreover, inhibition of CPE decreases ictal discharges in vivo. This study reveals a complex landscape of homeostatic changes affecting the synaptic release machinery , differentially at inhibitory and excitatory terminals. We propose a novel homeostatic presynaptic mechanism which may impact release timing rather than synaptic strength.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_13:34:07.647.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liam McDonnell
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-30 07:49:08	ID requested
⏵ 1	2021-09-09 13:34:08	announced

Vannini E, Restani L, Dilillo M, McDonnell LA, Caleo M, Marra V, Synaptic Vesicles Dynamics in Neocortical Epilepsy. Front Cell Neurosci, 14():606142(2020) [pubmed]

submitter keyword: Epilepsy, synaptosome, tetanus neurotoxin, TMT

Liam McDonnell
contact affiliation	Fondazione Pisana per la Scienza
contact email	l.a.mcdonnell@outlook.com
lab head
Liam McDonnell
contact affiliation	Fondazione Pisana per la Scienza
contact email	l.a.mcdonnell@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD022270

PRIDE project URI

• PRIDE
  1. PXD022270
     1. Label: PRIDE project
     2. Name: Synaptosome proteomics of tetanus neurotoxin-injected mice