PXD022264 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Isolation and characterization of equine uterine extracellular vesicles: a comparative methodological study |
Description | Extracellular vesicles (EVs) have been identified in the uterine fluid in different species and pointed as key players in the embryo-maternal dialogue, maternal recognition of pregnancy (MRP) and establishment of pregnancy. However, little is known about the uterine EVs in the mare. Therefore, the present study aimed at characterizing EVs from uterine lavage of cyclic mares by comparing five EVs isolation methods and the combination of them: (1) Ultracentrifugation (UC); (2) Concentration of lavage volume by Centricon ultrafiltration (CE); (3) the use of CE with different washing steps (PBS versus trehalose); (4) Size-exclusion chromatography with iZON-qEV columns to yield high-purity EVs and (5) a combination of the methods with best results based on EVs yield, purity, and protein cargo profiles. Transmission electron microscopy and Western blotting confirmed the isolation of EVs by all methods but with quantitative and qualitative differences. Mass spectrometry provided different protein profiles among methods with marked differences in the number of proteins and protein classes. Our results indicate that the combination of CE/trehalose/iZON/UC is an optimal method to isolate equine uterine EVs with good yield and purity that can be applied in future studies to determine the role of uterine EVs during MRP in the mare. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_13:16:08.078.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Fröhlich |
SpeciesList | scientific name: Equus caballus; NCBI TaxID: 9796; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-30 04:14:54 | ID requested | |
⏵ 1 | 2021-09-09 13:16:08 | announced | |
Publication List
Almi, ñ, ana C, Rudolf Vegas A, Tekin M, Hassan M, Uzbekov R, Fr, ö, hlich T, Bollwein H, Bauersachs S, Isolation and Characterization of Equine Uterine Extracellular Vesicles: A Comparative Methodological Study. Int J Mol Sci, 22(2):(2021) [pubmed] |
Keyword List
submitter keyword: Extracellular vesicles, exosomes, uterus, mare, Equus caballus, EVs protein cargo, uterine fluid, uterine lavage |
Contact List
Thomas Fröhlich |
contact affiliation | Ludwig-Maximilians-Universität München Genzentrum Laboratory for Functional Genome Analysis Feodor-Lynen-Str. 25 D-81377 München |
contact email | frohlich@genzentrum.lmu.de |
lab head | |
Thomas Fröhlich |
contact affiliation | Laboratory for Functional Genome Analysis Gene Center Munich Ludwig-Maximilians-Universität Munich (LMU) Feodor-Lynen-Str. 25 81377 Munich Germany |
contact email | frohlich@genzentrum.lmu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD022264 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022264
- Label: PRIDE project
- Name: Isolation and characterization of equine uterine extracellular vesicles: a comparative methodological study