PXD022249

PXD022249 is an original dataset announced via ProteomeXchange.

Title	Root membrane ubiquitinome under osmotic stress reveals specific post-translational modification patterns
Description	Osmotic stress is detrimental for the plant which survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification (PTM) in osmotic mediated stress. Plants use the ubiquitin proteasome system to modulate protein contents required to respond to and tolerate adverse growth conditions and a role for ubiquitin to mediate endocytosis and trafficking of plant plasma membrane proteins has recently emerged. In this study, using the K-Ɛ-GG antibody enrichment method integrated with high-resolution mass spectrometry, a list of 786 ubiquitinated lysine (K-Ub) residues in 451 proteins of which 50 % were transmembrane proteins was compiled for Arabidopsis root membrane proteins, increasing the database of ubiquitinated substrates in plants. Such analysis allowed to evidence specific ubiquitination motifs, a role for ubiquitination in the internalization and sorting of cargo proteins and ubiquitination dialog with other post-translational modifications (PTMs). In silico interactomics analysis allowed to pinpoint two E2 ligases, UBC32 and UBC34 targeting membrane proteins and putatively involved in response to osmotic stress. The simultaneous quantification of proteome and ubiquitinome after plant treatment with mannitol, suggested a minor role for ubiquitination in protein degradation. Then, this study revealed that a major plasma membrane aquaporin (PIP2;1) harbors two ubiquitination sites that dialog with N-terminal acetylation and C-terminal phosphorylation, to putatively limit PIP2;1 degradation and to favor its internalization contributing to a decreased root hydraulic conductivity (Lpr) while maintaining its cellular abundance.
HostingRepository	PRIDE
AnnounceDate	2022-05-27
AnnouncementXML	Submission_2022-05-27_05:23:09.663.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	sonia hem
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-29 08:17:53	ID requested
⏵ 1	2022-05-27 05:23:10	announced

Berger N, Demolombe V, Hem S, Rofidal V, Steinmann L, Krouk G, Crabos A, Nacry P, Verdoucq L, Santoni V, Root Membrane Ubiquitinome under Short-Term Osmotic Stress. Int J Mol Sci, 23(4):(2022) [pubmed]

submitter keyword: Aquaporin, mass spectrometry, osmotic stress, ubiquitination, post-translational modification

santoni veronique
contact affiliation	BPMP, CNRS, INRAE, Institut Agro, Univ Montpellier, 34060 Montpellier, France
contact email	veronique.santoni@inrae.fr
lab head
sonia hem
contact affiliation	INRA
contact email	sonia.hem@supagro.inra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022249
     1. Label: PRIDE project
     2. Name: Root membrane ubiquitinome under osmotic stress reveals specific post-translational modification patterns