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PXD022247

PXD022247 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic profiling of FAC-sorted ex vivo and in vitro expanded Sca-1+cells derived from murine skeletal muscle
DescriptionStem cells antigen-1 (Sca-1) is an 18-kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein that has widely been used for isolation of murine hematopoietic stem cells (HSCs). However, recently several studies have reported the expression of this protein in non-HSCs such as the liver, skin and muscles among others. In the current study, we used fluorescence-activated cell sorting (FACS) technique to sort Sca-1+ stem cells from mouse hindlimb muscles. The Sca-1+ cells were divided into two sets first set of Sca-1+ cells were processed for protein extraction immediately after sorting (ex vivo set) while the second set of samples, referred to as in vitro set, were expanded in cell culture after sorting and harvested after two passage and then processed for protein extraction. A high-resolution proteome map of the ex vivo and in vitro sets was generated Orbitrap Fusion Tribrid Mass Spectrometer. In total, the analysis led to the identification of 5,581 protein groups.
HostingRepositoryPRIDE
AnnounceDate2021-06-21
AnnouncementXMLSubmission_2021-06-20_22:35:55.667.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSaketh Kapoor
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-29 08:17:53ID requested
12021-06-20 22:35:56announced
Publication List
Kapoor S, Subba P, Shenoy P S, Bose B, Cells (In vitro), Both Isolated From Murine Skeletal Muscle Tissue. Stem Cell Rev Rep, 17(5):1754-1767(2021) [pubmed]
Keyword List
submitter keyword: Stem Cell Antigen-1 (Sca-1), regenerative stem cells, cell therapy, stem cells proteomics, mass spectrometry-based proteomics
Contact List
Dr Bipasha Bose
contact affiliationAssociate Professor, Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya Deemed to be University Mangalore 575 018 INDIA
contact emailbipasha.bose@gmail.com
lab head
Saketh Kapoor
contact affiliationStem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya Deemed to be University
contact emailkapoor.saketh@gmail.com
dataset submitter
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Dataset FTP location
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