PXD022247 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic profiling of FAC-sorted ex vivo and in vitro expanded Sca-1+cells derived from murine skeletal muscle |
Description | Stem cells antigen-1 (Sca-1) is an 18-kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein that has widely been used for isolation of murine hematopoietic stem cells (HSCs). However, recently several studies have reported the expression of this protein in non-HSCs such as the liver, skin and muscles among others. In the current study, we used fluorescence-activated cell sorting (FACS) technique to sort Sca-1+ stem cells from mouse hindlimb muscles. The Sca-1+ cells were divided into two sets first set of Sca-1+ cells were processed for protein extraction immediately after sorting (ex vivo set) while the second set of samples, referred to as in vitro set, were expanded in cell culture after sorting and harvested after two passage and then processed for protein extraction. A high-resolution proteome map of the ex vivo and in vitro sets was generated Orbitrap Fusion Tribrid Mass Spectrometer. In total, the analysis led to the identification of 5,581 protein groups. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-21 |
AnnouncementXML | Submission_2021-06-20_22:35:55.667.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Saketh Kapoor |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-29 08:17:53 | ID requested | |
⏵ 1 | 2021-06-20 22:35:56 | announced | |
Publication List
Kapoor S, Subba P, Shenoy P S, Bose B, Cells (In vitro), Both Isolated From Murine Skeletal Muscle Tissue. Stem Cell Rev Rep, 17(5):1754-1767(2021) [pubmed] |
Keyword List
submitter keyword: Stem Cell Antigen-1 (Sca-1), regenerative stem cells, cell therapy, stem cells proteomics, mass spectrometry-based proteomics |
Contact List
Dr Bipasha Bose |
contact affiliation | Associate Professor, Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya Deemed to be University Mangalore 575 018 INDIA |
contact email | bipasha.bose@gmail.com |
lab head | |
Saketh Kapoor |
contact affiliation | Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya Deemed to be University |
contact email | kapoor.saketh@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022247
- Label: PRIDE project
- Name: Proteomic profiling of FAC-sorted ex vivo and in vitro expanded Sca-1+cells derived from murine skeletal muscle