PXD022246

PXD022246 is an original dataset announced via ProteomeXchange.

Title	Exocyst Subunit Exo70B2 is Linked to Immune Signalling and Autophagy
Description	During the immune response, activation of the secretory pathway is key to mount an effective response, while gaging its output is important to maintain cellular homeostasis. Exo70 subunit of the exocyst functions as a spatiotemporal regulator by mediating numerous interactions with proteins and lipids. However, a molecular understanding of the exocyst regulation remains challenging. We show that the Exo70B2 behaves as a bona fide exocyst subunit. Conversely, treatment with the salicylic acid (SA) defence hormone analogue Benzothiadiazole (BTH), or the immunogenic peptide flg22, induced Exo70B2 transport into the vacuole. We reveal that Exo70B2 interacts with ATG8 via two ATG8-interacting motives (AIMs) and its transport into the vacuole was dependent on autophagy. In line with its role in immunity, we discovered that Exo70B2 interacted with and was phosphorylated by the kinase MPK3. Mimicking phosphorylation had a dual impact on Exo70B2; first, by inhibiting localization at sites of active secretion, and second, it increased the interaction with ATG8. Phosphonull variants displayed higher Effector-Triggered Immunity (ETI) and were hypersensitive to BTH, which induce secretion and autophagy. Our results suggest a molecular mechanism by which phosphorylation diverts Exo70B2 from the secretory into the autophagy pathway for its degradation, to dampen secretory activity.
HostingRepository	PRIDE
AnnounceDate	2021-03-01
AnnouncementXML	Submission_2021-03-01_03:23:51.871.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steffen Neumann
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-29 06:34:35	ID requested
⏵ 1	2021-03-01 03:23:52	announced

Brillada C, Teh OK, Ditengou FA, Lee CW, Klecker T, Saeed B, Furlan G, Zietz M, Hause G, Eschen-Lippold L, Hoehenwarter W, Lee J, Ott T, Trujillo M, Exocyst subunit Exo70B2 is linked to immune signaling and autophagy. Plant Cell, 33(2):404-419(2021) [pubmed]

submitter keyword: Phosphorylation, Exo70B2, ATG8, vacuole, autophagy, signalling, secretion

Wolfgang Hoehenwarter
contact affiliation	Leibniz Institute of Plant Biochemistry Research Group Head Proteome Biology of Plant Interactions
contact email	Wolfgang.Hoehenwarter@ipb-halle.de
lab head
Steffen Neumann
contact affiliation	IPB Halle
contact email	sneumann@ipb-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022246
     1. Label: PRIDE project
     2. Name: Exocyst Subunit Exo70B2 is Linked to Immune Signalling and Autophagy