PXD022242

PXD022242 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry guided by transcriptomics reveals multiple classes of predicted antimicrobial peptides in Amaranthus tricolor
Description	Traditional medicinal plants are rich reservoirs of antimicrobial agents, including antimicrobial peptides (AMPs). Herein, Amaranthus tricolor AMPs predicted in silico are identified via proteomics profiling. Bottom-up proteomics identified seven novel peptides spanning three AMP classes including lipid transfer proteins, snakins and defensins. Characterization via top-down peptidomic analysis of Atr-SN1, Atr-DEF1, and Atr-LTP1 revealed unexpected proteolytic processing and enumerated disulfide bonds. These results highlight the potential for integrating AMP prediction algorithms with complementary -omics approaches to accelerate characterization of biologically relevant AMP peptidoforms.
HostingRepository	PRIDE
AnnounceDate	2021-04-08
AnnouncementXML	Submission_2021-04-08_06:22:45.042.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leslie Hicks
SpeciesList	scientific name: Amaranthus tricolor; NCBI TaxID: 29722;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-29 02:40:30	ID requested
⏵ 1	2021-04-08 06:22:46	announced

Moyer TB, Allen JL, Shaw LN, Hicks LM, Revealed by Prediction, Proteomics, and Mass Spectrometric Characterization. J Nat Prod, 84(2):444-452(2021) [pubmed]

submitter keyword: Antimicrobial peptides, Amaranthus tricolor, mass spectrometry, peptidomics

Leslie M. Hicks
contact affiliation	Department of Chemistry, University of North Carolina at Chapel Hiil, USA
contact email	lmhicks@unc.edu
lab head
Leslie Hicks
contact affiliation	University of North Carolina at Chapel Hill
contact email	lmhicks@unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022242
     1. Label: PRIDE project
     2. Name: Mass spectrometry guided by transcriptomics reveals multiple classes of predicted antimicrobial peptides in Amaranthus tricolor