PXD022231

PXD022231 is an original dataset announced via ProteomeXchange.

Title	The grain protein turnover atlas of hexaploid wheat Triticum aestivum
Description	Cellular protein abundance results from the relative rates of protein synthesis and protein degradation. Through combining in vivo stable isotope labelling and in-depth quantitative proteomics, we created a protein turnover atlas of wheat grain proteins during grain development. Our data demonstrate that protein turnover rates for 1447 unique wheat grain protein groups have an apparent spatiotemporal pattern that aids explanation of the 60% of variation in protein abundances that are not attributable to gene expression. Protein synthesis rates of individual proteins vary over 100 fold and degradation rates over 20 fold. Storage proteins have both higher synthesis and degradation rates than the overarching average rates of grain proteins in other functional categories, while those proteins involved in photosynthesis, DNA synthesis and glycolysis, by contrast, are house-keeping proteins that show low synthesis and degradation rates at all times. Approximately 20% of total grain ATP production through respiration is used for grain proteome biogenesis and maintenance, and the grain invests nearly half of this budget in storage protein synthesis alone. Degradation of storage proteins as a class of grain proteins also consumed a significant amount of the total ATP allocated to protein degradation processes. This analysis suggests that 20% of newly synthesized storage proteins are turned over rather than stored suggesting that this process is not energetically optimal. This approach to measure protein turnover rates at the proteome scale shows how different functional categories of grain proteins accumulate, calculates the costs of futile cycling of protein turnover during wheat grain development and identifies the most and the least stable wheat grain proteins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:15.976.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HUI CAO
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	No PTMs are included in the dataset
Instrument	6550A iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-28 08:24:50	ID requested
1	2021-12-20 19:07:08	announced
⏵ 2	2023-11-14 08:51:16	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: grain, LC-MSMS, Protein turnover,Wheat, GC-MSMS

A.Harvey Millar
contact affiliation	ARC Centre of Excellence in Plant Energy Biology The University of Western Australia
contact email	harvey.millar@uwa.edu.au
lab head
HUI CAO
contact affiliation	The University of Western Australia
contact email	hui.cao@uwa.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD022231

PRIDE project URI

• PRIDE
  1. PXD022231
     1. Label: PRIDE project
     2. Name: The grain protein turnover atlas of hexaploid wheat Triticum aestivum