PXD022229

PXD022229 is an original dataset announced via ProteomeXchange.

Title	Early events of the symbiotic interaction between Oryza sativa and Nostoc punctiforme revealed by a quantitative proteomic approach.
Description	Plant-cyanobacteria symbiosis is considered one of the pivotal events in the history of life. In this symbiosis, the cyanobacterium provides to the plant fixed nitrogen compounds and plant hormones and, in return, the plant provides to the cyanobacterium fixed carbon. Despite the large knowledge in the physiology and ecology of plant-cyanobacteria symbioses, little is known about the molecular mechanisms involved in the crosstalk between partners. It has been shown recently that Nostoc punctiforme is able to stablish an endophytic symbiosis with Oryza sativa. This finding opens a door to explore this symbiotic interaction as a sustainable alternative to nitrogen fertilization of paddy fields. However, molecular mechanisms behind Oryza-Nostoc endosymbiosis are still not clarified. To gain further insights, an LC-MS/MS based label-free quantitative technique was used to evaluate the differential proteomics under N. punctiforme treatment vs. control plants at 1 day and 7 days. Differential expression profiling reveals a significant number of proteins to be down-regulated or missing in both partners, while others were more abundant or only expressed when both partners were in contact. In N. punctiforme, the differential protein expression was primarily connected to primary metabolism, signal transduction and perception, transport of substances and photosynthesis. In O. sativa, the differential protein expression was connected to a wide range of biological functions regulating carbon and nitrogen metabolism and response to biotic and abiotic stresses.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:37:32.424.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alvarez Nunez Consolacion
SpeciesList	scientific name: Nostoc punctiforme (strain ATCC 29133 / PCC 73102); NCBI TaxID: 63737; scientific name: O. sativa Indica Group; NCBI TaxID: 39946;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-28 08:13:09	ID requested
1	2023-03-10 22:06:37	announced
⏵ 2	2023-11-14 08:37:33	announced	2023-11-14: Updated project metadata.

Á, lvarez C, Brenes-, Á, lvarez M, Molina-Heredia FP, Mariscal V, Quantitative Proteomics at Early Stages of the Symbiotic Interaction Between Oryza sativa and Nostoc punctiforme Reveals Novel Proteins Involved in the Symbiotic Crosstalk. Plant Cell Physiol, 63(10):1433-1445(2022) [pubmed]

submitter keyword: SWATH-MS., Oryza,Plant-cyanobacteria Symbiosis, Nostoc

Vicente Mariscal
contact affiliation	Instituto de Bioquimica Vegetal y Fotosintesis CSIC Americo Vespucio 49. 41092. Seville (Spain).
contact email	vicente.mariscal@ibvf.csic.es
lab head
Alvarez Nunez Consolacion
contact affiliation	CSIC
contact email	consolacion@ibvf.csic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022229
     1. Label: PRIDE project
     2. Name: Early events of the symbiotic interaction between Oryza sativa and Nostoc punctiforme revealed by a quantitative proteomic approach.