PXD022220

PXD022220 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics analysis of Mycoplasma pneumoniae identifies potential macrolide resistance determinants
Description	Mycoplasma pneumoniae is one of the leading causes of community-acquired pneumonia in children and adolescents. Because of the wide application of macrolides in clinical treatment, macrolide-resistant M. pneumoniae strains have become increasingly common worldwide. However, the molecular mechanisms underlying drug resistance in M. pneumoniae are poorly understood. In the present work, we analyzed the whole proteomes of macrolide-sensitive and macrolide-resistant strains of M. pneumoniae using a tandem mass tag-labeling quantitative proteomic technique. In total, 165 differentially expressed proteins were identified, of which 80 were upregulated and 85 were downregulated in the drug-resistant strain compared with the sensitive strain. Functional analysis revealed that these proteins were predominantly involved in protein and peptide biosynthesis processes, the ribosome, and transmembrane transporter activity, which implicates them in the mechanism(s) of resistance of M. pneumoniae to macrolides. Our results provide new insights into drug resistance in M. pneumoniae and identify potential targets for further studies on resistance mechanisms in this bacterium.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:19:25.524.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022220
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jing Yin
SpeciesList	scientific name: Mycoplasma pneumoniae; NCBI TaxID: 2104;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-28 03:12:12	ID requested
1	2021-02-25 02:19:59	announced
⏵ 2	2024-10-22 05:19:27	announced	2024-10-22: Updated project metadata.

10.1186/s13568-021-01187-8;

Li S, Xue G, Zhao H, Feng Y, Yan C, Cui J, Xie X, Yuan J, Quantitative proteomics analysis of Mycoplasma pneumoniae identifies potential macrolide resistance determinants. AMB Express, 11(1):26(2021) [pubmed]

10.6019/PXD022220;

submitter keyword: drug resistance,Mycoplasma pneumoniae, quantitative proteomic technique, differentially expressed proteins, whole proteomes

Yuan Jing
contact affiliation	department of Bacteriology Capital Institute of Pediatrics
contact email	yuanjing6216@163.com
lab head
Jing Yin
contact affiliation	Beijing BioTech Pack Technology Company
contact email	tech.sales@biotech-pack.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022220
     1. Label: PRIDE project
     2. Name: Quantitative proteomics analysis of Mycoplasma pneumoniae identifies potential macrolide resistance determinants