PXD022214 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Fibrosis associated changes detected in the proteome of surface epithelium overlying Crohn’s Disease Strictures |
Description | Background and aims: Intestinal fibrosis is a common complication of Crohn’s disease (CD). It is characterised by an excessive accumulation of fibroblasts differentiating into activated myofibroblasts secreting excessive extracellular matrix. The potential role of the intestinal epithelium in this fibrotic process remains poorly defined. Methods: A total of 113 CD and control subjects were studied. We performed a pilot proteomic study comparing the proteome of surface epithelium isolated by laser capture microdissection in normal and fibrotic zones of resected ileal CD strictures (n=5). Selected proteins were validated by immunohistochemistry (IHC) in colonic and ileal samples of stricturing CD patients (n=44), pure inflammatory CD (n=29) and control subjects (n=40). Functional assays with cell lines cultures and a fibroblast to myofibroblast differentiation model were used to assess the role of the highlighted epithelial proteins in CD fibrosis. Results: Proteomic study revealed an endoplasmic reticulum (ER) stress and unfolded protein response (UPR) markers increase in the epithelium overlying ileal fibrotic strictures, involving Anterior gradient protein 2 homolog (AGR2) and Binding immunoglobulin protein (BiP). This was confirmed by IHC. The ER stress induction in intestinal epithelial cells increased AGR2 as well as BiP expression and led to an extracellular secreted AGR2. A fibroblast to myofibroblast differentiation was obtained with the supernatant of intestinal epithelial cells pre-conditioned by ER stress and with recombinant AGR2. Conclusions: AGR2 and other ER stress markers are increased within the intestinal epithelium overlying fibrotic strictures and might contribute to profibrotic signals involved in CD fibrosis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-27 |
AnnouncementXML | Submission_2022-05-27_05:23:07.551.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marie-Alice Meuwis |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-28 01:29:33 | ID requested | |
⏵ 1 | 2022-05-27 05:23:08 | announced | |
Publication List
Vieujean S, Hu S, Bequet E, Salee C, Massot C, Bletard N, Pierre N, Quesada Calvo F, Baiwir D, Mazzucchelli G, De Pauw E, Coimbra Marques C, Delvenne P, Rieder F, Louis E, Meuwis MA, Potential Role of Epithelial Endoplasmic Reticulum Stress and Anterior Gradient Protein 2 Homologue in Crohn's Disease Fibrosis. J Crohns Colitis, 15(10):1737-1750(2021) [pubmed] |
Keyword List
submitter keyword: Inflammatory bowel disease, ileum, fibrostenosis, surface epithelium, label free proteomics |
Contact List
Edouard LOUIS |
contact affiliation | Translational gastroenterology, GIGA institute, University of Liege ULiege Belgium and Hepatogastro entreology and digestive oncology University hospitalof Liège, CHU Liège |
contact email | Edouard.Louis@Uliege.be |
lab head | |
Marie-Alice Meuwis |
contact affiliation | translational gastroenterology laboratory University of Liege, GIGA institute: university hospital CHU Liège, BE |
contact email | marie-alice.meuwis@uliege.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022214
- Label: PRIDE project
- Name: Fibrosis associated changes detected in the proteome of surface epithelium overlying Crohn’s Disease Strictures