PXD022203

PXD022203 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis on COE inhibiting vasculogenic mimicry in hepatocellular carcinoma
Description	New strategies and drugs are urgently needed to improve the treatment of hepatocellular carcinoma (HCC). Vasculogenic mimicry (VM) has been elucidated being associated with the progression of HCC and anti-VM could be a promising strategy. Celastrus orbiculatus extract (COE), a mixture of 11 terpenoids isolated from the Chinese Herb Celastrus Orbiculatus Vine, has been elucidated to be able to disrupt VM formation in HCC. This study aims to dissect and identify the potential targets of COE on anti-VM formation both in vitro and in vivo that are distinct from our previous study. Proteomics analysis was used to identify differential proteins in HCC cells treated with or without COE. Cells invasion was examined using Transwell. Matrigel was used to establish a 3-D culture condition for VM formation in vitro. RT-PCR and Western Blot were used to examine changes of mRNA and protein respectively. Clinical resected samples were applied to confirm association between VM formation and identified targets. Subcutaneous xenograft tumor model was established to observe tumor growth and VM formation in vivo. PAS-CD34 dual staining was used to detect VM in vivo. A total of 194 proteins were identified to be differentially expressed in HCC cells treated with or without COE. In the 93 down-regulated proteins EphA2 stood out to be regulated on both RNA and protein level. Disruption EphA2 using COE or NVP inhibited VM formation and decreased VM associated biomarkers. In xenograft mouse model, COE inhibited tumor growth and VM formation via down-regulating EphA2. Taken together, our results indicate that COE could be used in HCC treatment because of its promising anti-VM effect.
HostingRepository	PRIDE
AnnounceDate	2021-05-03
AnnouncementXML	Submission_2021-05-02_23:25:33.120.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CHEN JUE
SpeciesList	scientific name: Celastrus; NCBI TaxID: 85180;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-27 02:26:17	ID requested
⏵ 1	2021-05-02 23:25:33	announced

Chu Z, Shi X, Chen G, He X, Qian Y, Wang H, Tao L, Liu Y, Jiang W, Chen J, COE Inhibits Vasculogenic Mimicry by Targeting EphA2 in Hepatocellular Carcinoma, a Research Based on Proteomics Analysis. Front Pharmacol, 12():619732(2021) [pubmed]

submitter keyword: EphA2

hepatocellular carcinoma

proteomics

vasculogenic mimicry

cancer treatment

Jue Chen
contact affiliation	Institution of Integrated Traditional Chinese and Western Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, China
contact email	1019924551@qq.com
lab head
CHEN JUE
contact affiliation	Yangzhou University
contact email	1019924551@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022203
     1. Label: PRIDE project
     2. Name: Proteomics analysis on COE inhibiting vasculogenic mimicry in hepatocellular carcinoma