PXD022200

PXD022200 is an original dataset announced via ProteomeXchange.

Title	Astrocyte conditioned media-dependent Phosphatidylinositol 3-kinase interactome in mouse hippocampal neuron cell line Ht22
Description	Astrocytes can support neuronal survival through a range of secreted signals that protect against neurotoxicity, oxidative stress, and apoptotic cascades. To identify proteins contributing to protective intracellular neuronal signalling originating from astrocytes, endogenous PI3K was immunoprecipitated from Ht22 cells exposed to primary astrocyte conditioned media (ACM) or cell free media (CFM), followed by iTRAQ-based quantitative proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_13:14:37.227.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Declan Williams
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-pyridylethyl-L-cysteine; phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-27 02:26:04	ID requested
⏵ 1	2021-09-09 13:14:37	announced

Alqawlaq S, Livne-Bar I, Williams D, D'Ercole J, Leung SW, Chan D, Tuccitto A, Datti A, Wrana JL, Corbett AH, Schmitt-Ulms G, Sivak JM, An endogenous PI3K interactome promoting astrocyte-mediated neuroprotection identifies a novel association with RNA-binding protein ZC3H14. J Biol Chem, 296():100118(2021) [pubmed]

submitter keyword: Astrocyte, brain, neuron, neuroprotection, immunoprecipitation, interactome, orbitrap, Phosphatidylinositol 3-kinase, PI3K, iTRAQ

Gerold Schmitt-Ulms
contact affiliation	Tanz Centre for Research in Neurodegenerative Diseases, Faculty of Medicine, University of Toronto
contact email	g.schmittulms@utoronto.ca
lab head
Declan Williams
contact affiliation	University of Toronto
contact email	gwillym.williams@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD022200

PRIDE project URI

• PRIDE
  1. PXD022200
     1. Label: PRIDE project
     2. Name: Astrocyte conditioned media-dependent Phosphatidylinositol 3-kinase interactome in mouse hippocampal neuron cell line Ht22