PXD022199

PXD022199 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of a new Cas9-targeting PROTAC molecule
Description	Scientists have developed several methods to control the activity of Cas protein in a timely way and hence reduce off-target effects, including anti-CRISPR proteins and small molecule inhibitors. The PROTAC is a new conception of using the natural ubiquitin-proteasome system to degrade the protein of interest in drug design and development. Here, we engineered Cas proteins (Cas9, Cas12, and Cas13) by inserting a Phe-Cys-Pro-Phe (FCPF) amino acid sequence (also known as the π-clamp system) and demonstrate that the modified CasFCPF proteins could be labeled in live cells by perfluoroaromatics carrying FITC fluorophore or were degraded by perfluoroaromatics-functionalized PROTAC (PROTAC-FCPF). Proteome-wide analysis of PROTAC-FCPF-mediated Cas9FCPF protein degradation revealed high target specificity, suggesting a wide application of chemically induced proximity in combination with site-specific protein modification in the regulation of protein stability, activity and functionality.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:09:33.490.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georg Tascher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-27 02:25:52	ID requested
1	2022-10-18 08:38:55	announced
⏵ 2	2023-11-14 08:09:35	announced	2023-11-14: Updated project metadata.

Gama-Brambila RA, Chen J, Dabiri Y, Tascher G, N, ě, mec V, M, ü, nch C, Song G, Knapp S, Cheng X, A Chemical Toolbox for Labeling and Degrading Engineered Cas Proteins. JACS Au, 1(6):777-785(2021) [pubmed]

submitter keyword: CRISPR-Cas9, Qexactive HF, PROTAC, LC-MS/MS

Xinlai Cheng
contact affiliation	Buchmann Institute for Molecular Life Sciences, Pharmaceutical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Strasse 15. R. 3.652, D-60438 Frankfurt am Main.
contact email	cheng@pharmchem.uni-frankfurt.de
lab head
Georg Tascher
contact affiliation	Institute of Biochemistry II, Goethe University Hospital Frankfurt/Main, Germany
contact email	tascher@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD022199

PRIDE project URI

• PRIDE
  1. PXD022199
     1. Label: PRIDE project
     2. Name: Proteomics analysis of a new Cas9-targeting PROTAC molecule