PXD022184

PXD022184 is an original dataset announced via ProteomeXchange.

Title	The KdmB-EcoA-RpdA-SntB chromatin complex binds regulatory genes and coordinates fungal development with mycotoxin synthesis
Description	Chromatin complexes control a vast number of epigenetic developmental processes. Filamentous fungi present an important clade of microbes with poor understanding of underlying epigenetic mechanisms. Here, we describe a chromatin binding complex in the fungus Aspergillus nidulans composing of a H3K4 histone demethylase KdmB, a cohesin acetyltransferase (EcoA), a histone deacetylase (RpdA) and a histone reader/E3 ligase protein (SntB). In vitro and in vivo evidence demonstrate that this KERS complex is assembled from the EcoA-KdmB and SntB-RpdA heterodimers. KdmB and SntB play opposing roles in regulating the cellular levels and stability of EcoA, as KdmB prevents SntB-mediated degradation of EcoA. The KERS complex is recruited to transcription initiation start sites at active core promoters exerting promoter-specific transcriptional effects. Interestingly, deletion of any one of the KERS subunits results in a common negative effect on morphogenesis and production of secondary metabolites, molecules important for niche securement in filamentous fungi. Consequently, the entire mycotoxin sterigmatocystin gene cluster is downregulated and asexual development is reduced in the four KERS mutants. The elucidation of the recruitment of epigenetic regulators to chromatin via the KERS complex provides the first mechanistic, chromatin-based understanding of how development is connected with small molecule synthesis in fungi.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:33:35.856.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ozgur Bayram
SpeciesList	scientific name: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans); NCBI TaxID: 227321;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 03:58:20	ID requested
1	2022-10-13 15:40:38	announced
⏵ 2	2023-11-14 07:33:39	announced	2023-11-14: Updated project metadata.

Karahoda B, Pardeshi L, Ulas M, Dong Z, Shirgaonkar N, Guo S, Wang F, Tan K, Sarikaya-Bayram Ö, Bauer I, Dowling P, Fleming AB, Pfannenstiel BT, Luciano-Rosario D, Berger H, Graessle S, Alhussain MM, Strauss J, Keller NP, Wong KH, Bayram Ö, The KdmB-EcoA-RpdA-SntB chromatin complex binds regulatory genes and coordinates fungal development with mycotoxin synthesis. Nucleic Acids Res, 50(17):9797-9813(2022) [pubmed]

submitter keyword: A. nidulans, LC-MSMS

Ozgur Bayram
contact affiliation	Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland
contact email	ozgur.bayram@mu.ie
lab head
Ozgur Bayram
contact affiliation	Dr.
contact email	ozgur.bayram@mu.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022184
     1. Label: PRIDE project
     2. Name: The KdmB-EcoA-RpdA-SntB chromatin complex binds regulatory genes and coordinates fungal development with mycotoxin synthesis