PXD022182

PXD022182 is an original dataset announced via ProteomeXchange.

Title	Proteomics-based approach reveals the involvement of SERPINB9 in recurrent and relapsed multiple myeloma
Description	Multiple myeloma (MM) is a common hematological malignancy with poorly understood recurrence and relapse mechanisms. Notably, bortezomib resistance leading to relapse makes MM treatment significantly challenging. To clarify the drug resistance mechanism, we employed a quantitative proteomics approach to identify differentially expressed protein candidates implicated in bortezomib-resistant recurrent and relapsed MM (RRMM). Bone marrow biopsy specimens from five patients newly diagnosed with MM (NDMM) were compared with those from five patients diagnosed with bortezomib-resistant RRMM using tandem mass tag-mass spectrometry (TMT-MS). Subcellular localization and functional classification of the differentially expressed proteins were determined by gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and hierarchical clustering. Top candidates identified were validated with parallel reaction monitoring (PRM) analysis using tissue samples from 11 NDMM and 8 RRMM patients, followed by comparison with the NCBI Gene Expression Omnibus (GEO) dataset of 10 MM patients and 10 healthy controls (Accession No.: GSE80608). Thirty-four differentially expressed proteins in RRMM, including proteinase inhibitor 9 (SERPINB9) were identified by TMT-MS. Subsequent functional enrichment analyses of the identified protein candidates indicated their involvement in regulating cellular metabolism, apoptosis, programmed cell death, lymphocyte-mediated immunity, and defense response pathways in RRMM. The top protein candidate SERPINB9 was confirmed by PRM analysis as well as by comparison with an NCBI GEO dataset. We elucidated the proteome landscape of bortezomib-resistant RRMM and identified SERPINB9 as a promising novel therapeutic target. Our results provide a resource for future studies on the mechanism of RRMM.
HostingRepository	PRIDE
AnnounceDate	2021-03-03
AnnouncementXML	Submission_2021-03-03_01:20:26.134.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lina Quan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 02:49:45	ID requested
⏵ 1	2021-03-03 01:20:26	announced

Chen Y, Quan L, Jia C, Guo Y, Wang X, Zhang Y, Jin Y, Liu A, Proteomics-Based Approach Reveals the Involvement of SERPINB9 in Recurrent and Relapsed Multiple Myeloma. J Proteome Res, 20(5):2673-2686(2021) [pubmed]

submitter keyword: multiple myeloma

recurrence and relapse

proteomics

parallel reaction monitoring

SERPINB9

Aichun Liu
contact affiliation	Hematology Department, Harbin Medical University Cancer Hospital
contact email	aichun2002@hotmail.com
lab head
Lina Quan
contact affiliation	Harbin Medical University Cancer Hospital
contact email	alina1123@yeah.net
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022182
     1. Label: PRIDE project
     2. Name: Proteomics-based approach reveals the involvement of SERPINB9 in recurrent and relapsed multiple myeloma