PXD022178

PXD022178 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Foxc1-interacting proteins in HEK293T cells
Description	Foxc1 has been identified to participate in regulating genes expression directly by binding their promoters as well as playing various roles in signal transduction within the cells. In this study, we found that, in F9 Embryonal Carcinoma cells, Foxc1 repressed the promoter’s transcription activity by binding the region of 1Kb upstream of Transcription Start Site within Nanog promoter.To understand more functions of Foxc1, streptavidin-biotin affinity purification combined with Liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) analysis was carried out.Bioinformatic analysis identified 360 proteins which may act as Foxc1 interactors.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_13:10:04.729.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongni Xue
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 02:49:21	ID requested
⏵ 1	2021-09-09 13:10:08	announced

Xue H, Liu F, Ai Z, Ke J, Yu M, Chen B, Guo Z, FOXC1 Downregulates Nanog Expression by Recruiting HDAC2 to Its Promoter in F9 Cells Treated by Retinoic Acid. Int J Mol Sci, 22(5):(2021) [pubmed]

submitter keyword: LC-MSMS, Foxc1

Zekun Guo
contact affiliation	1. College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi, China; 2. Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, China
contact email	gzk@nwsuaf.edu.cn
lab head
Hongni Xue
contact affiliation	College of Veterinary Medicine, Northwest A&F University
contact email	605422169@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD022178

PRIDE project URI

• PRIDE
  1. PXD022178
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Foxc1-interacting proteins in HEK293T cells