PXD022177

PXD022177 is an original dataset announced via ProteomeXchange.

Title	Chronically elevated exogenous glucose elicits antipodal effects on the proteome signature of differentiating human pancreatic progenitors
Description	The past decade revealed that cell identity changes, such as dedifferentiation or transdifferentiation, accompany the insulin-producing β-cell decay in most diabetes conditions. Mapping and controlling the mechanisms governing these processes is thus extremely valuable for managing the disease progression. Extracellular glucose is known to impact cell identity by impacting the redox balance. Here we use global proteomics and pathway analysis to map the response of differentiating human pancreatic progenitors to chronically increased in vitro glucose levels. We show that exogenous high glucose levels impact different protein subsets in a concentration-dependent manner. In contrast, regardless of concentration, high exogenous glucose elicits an antipodal effect on the proteome landscape, inducing both beneficial and detrimental changes in regard to achieving the desired islet cell fingerprint. Furthermore, we identified that only a subgroup of these effects and pathways are regulated by changes in redox balance. Our study highlights a complex yin-yang action of exogenous glucose on differentiating pancreas progenitors with a distinct proteome signature.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_02:50:04.807.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Aga Legøy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 02:49:17	ID requested
⏵ 1	2021-11-03 02:50:05	announced

Ghila L, Leg, ø, y TA, Mathisen AF, Abadpour S, Paulo JA, Scholz H, R, æ, der H, Chera S, Chronically Elevated Exogenous Glucose Elicits Antipodal Effects on the Proteome Signature of Differentiating Human iPSC-Derived Pancreatic Progenitors. Int J Mol Sci, 22(7):(2021) [pubmed]

submitter keyword: Human, iPSCs, glucose

Simona Chera
contact affiliation	Department of Clinical Science, University of Bergen, Norway
contact email	simona.chera@uib.no
lab head
Thomas Aga Legøy
contact affiliation	University of Bergen
contact email	tle088@uib.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022177
     1. Label: PRIDE project
     2. Name: Chronically elevated exogenous glucose elicits antipodal effects on the proteome signature of differentiating human pancreatic progenitors