PXD022174

PXD022174 is an original dataset announced via ProteomeXchange.

Title	Characterizations of molecularly distinct subpopulations of conditioned medium derived extracellular vesicles prior to be used as medicinal product
Description	Mesenchymal stromal cells (MSCs) derived EVs are greatly studied in disease models but lack of manifested characterization rather than generic EVs characterization in different EV subpopulations, greatly limits our ability to bring the most reliable EV types into our studies. We provide a comparative evaluation between three most well-reputed EVs subpopulations isolated by ultracentrifuge (EV-110K), sucrose gradient (EV-S110K), and 20,000g centrifugation only (EV-20K). We delved into purity, membrane perfectness, genuine content and apoptotic markers as prior factors need to be checked before functional application. Based on our observations, EV-SUC confirmed higher membrane integrity compare to EV-20K (P<0.01) and EV-110K (P<0.05) and carried the parental content like as acetylcholinesterase (AChE), however it requires to be adjusted in yield of purity. Based on these findings, EV-S110K as selected EV, also showed promising functional results on healthy and diabetic islet derived cell. Proteome profiling also confirmed distinct protein content of these three sub-populations. Our results attempt to stand up for the necessity of determining the most practical EV subpopulations prior to check its functional tests in in vitro models or even in disease models.
HostingRepository	PRIDE
AnnounceDate	2026-03-23
AnnouncementXML	Submission_2026-03-22_17:46:43.771.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yunqi Wu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 02:49:02	ID requested
⏵ 1	2026-03-22 17:46:45	announced

10.1002/jex2.159;

Abyadeh M, Mirshahvaladi S, Kashani SA, Paulo JA, Amirkhani A, Mehryab F, Seydi H, Moradpour N, Jodeiryjabarzade S, Mirzaei M, Gupta V, Shekari F, Salekdeh GH, Proteomic profiling of mesenchymal stem cell-derived extracellular vesicles: Impact of isolation methods on protein cargo. J Extracell Biol, 3(6):e159(2024) [pubmed]

submitter keyword: extracellular vesicles,mesenchymal stromal cells, subpopulations

Ghasem Hosseini Salekdeh
contact affiliation	Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
contact email	hosseini.salekdeh@mq.edu.au
lab head
Yunqi Wu
contact affiliation	Macquarie University
contact email	yunqi.wu@mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD022174

PRIDE project URI

• PRIDE
  1. PXD022174
     1. Label: PRIDE project
     2. Name: Characterizations of molecularly distinct subpopulations of conditioned medium derived extracellular vesicles prior to be used as medicinal product