PXD022172

PXD022172 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Characterization of m-lipin using HDX-MS
Description	This project consists of two experiments. The first is mapping the binding interface between the isolated m-lip domain of mouse lipin and liposomes. The second experiments is mapping the binding interface between full length mouse lipin and liposomes. Looking at the isolated m-lip domain, we found that residues 470-490 and 500-550 showed decreases in exchange upon liposome binding. The full-length lipin experiment saw decreases in exchnage in these same regions, as well as in the very C-terminus and very N-terminus regions of the protein. An order-disorder experiment was done on full length lipin where the protein was exposed to a short pulse of deuterium and compared to the fully-deuterated protein. In this instance, we established that the majority of the protein is relatively disordered and does not have secondary structure with high stability
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:23:28.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Burke
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	impact HD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 02:48:50	ID requested
1	2021-06-29 13:51:58	announced
2	2021-08-26 04:31:59	announced	2021-08-26: Updated publication reference for PubMed record(s): 34354069.
⏵ 3	2024-10-22 05:23:29	announced	2024-10-22: Updated project metadata.

Publication List

10.1038/s41467-021-24929-5;
Gu W, Gao S, Wang H, Fleming KD, Hoffmann RM, Yang JW, Patel NM, Choi YM, Burke JE, Reue K, Airola MV, The middle lipin domain adopts a membrane-binding dimeric protein fold. Nat Commun, 12(1):4718(2021) [pubmed]

10.1038/s41467-021-24929-5;

Gu W, Gao S, Wang H, Fleming KD, Hoffmann RM, Yang JW, Patel NM, Choi YM, Burke JE, Reue K, Airola MV, The middle lipin domain adopts a membrane-binding dimeric protein fold. Nat Commun, 12(1):4718(2021) [pubmed]

Keyword List

submitter keyword: m-lip domain, mass spectrometry,m-lipin, HDX-MS

submitter keyword: m-lip domain, mass spectrometry,m-lipin, HDX-MS

Contact List

John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
lab head
John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD022172
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD022172

PRIDE project URI

Repository Record List

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