PXD022171

PXD022171 is an original dataset announced via ProteomeXchange.

Title	Intestinal Differentiation Involves Cleavage of Histone H3 NTerminal Tails by Multiple Proteases
Description	The proteolytic cleavage of histone tails, also termed histone clipping, has been described as a mechanism for permanent removal of post-translational modifications (PTMs) from histone proteins. Such activity has been ascribed to ensure regulatory function in key cellular processes such as differentiation, senescence and transcriptional control for which different histone-specific proteases has been described. However, all these studies were exclusively performed using cell lines cultured in vitro and no clear evidences that histone clipping is regulated in vivo has been reported. Here we show that histone H3 N-terminal tails undergo extensive cleavage in the differentiated cells of the villi fraction of the mouse intestinal epithelium and we investigate histone PTM changes in full-length compared with clipped histone H3.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_13:49:03.539.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberta Noberini
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monomethylated residue; acetylated residue; N6; dimethylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 02:48:47	ID requested
⏵ 1	2021-03-04 13:49:04	announced

Ferrari KJ, Amato S, Noberini R, Toscani C, Fern, á, ndez-P, é, rez D, Rossi A, Conforti P, Zanotti M, Bonaldi T, Tamburri S, Pasini D, Intestinal differentiation involves cleavage of histone H3 N-terminal tails by multiple proteases. Nucleic Acids Res, 49(2):791-804(2021) [pubmed]

submitter keyword: histone post-translational modifications, clipping,

Tiziana Bonaldi
contact affiliation	European Institute of Oncology, Milan
contact email	tiziana.bonaldi@ieo.it
lab head
Roberta Noberini
contact affiliation	Istituto Europeo di Oncologia
contact email	roberta.noberini@ieo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD022171

PRIDE project URI

• PRIDE
  1. PXD022171
     1. Label: PRIDE project
     2. Name: Intestinal Differentiation Involves Cleavage of Histone H3 NTerminal Tails by Multiple Proteases