PXD022167

PXD022167 is an original dataset announced via ProteomeXchange.

Title	Isolation of aphid-Potato leafroll virus (PLRV) proteins complexes from viruliferous insects using affinity purification-mass spectrometry
Description	The control of insect borne disease is recognized as one of the major agricultural, animal and human health challenges of today. Viruses in the family Luteoviridae are phloem-limited, plant viruses that are vectored by aphids in a circulative manner. They are responsible for a wide-range of economically important disease in almost all staple food crops. In order to be transmitted, these viruses require species-specific passage of the pathogen across several membrane barriers within the insect. After the pathogen is ingested from the sap of an infected plant, the virus moves across and through the aphid gut into the hemoceol (insect blood) where it circulates until it reaches and enters the main or accessory salivary glands. From here, the pathogen is injected into a new plant host when the aphid feeds. The identification of insect proteins that interact with circulative plant viruses is technically challenging and a major goal for the plant vector biology field. Such information is critical to develop novel control strategies that block virus transmission by insects. In this study, we used affinity purification-high-resolution mass spectrometry (AP-MS) to rapidly capture and identify aphid proteins in complex with Potato leafroll virus (PLRV), a luteovirid transmitted by the green peach aphid (Myzus persicae), directly from viruliferous aphid tissue.
HostingRepository	PRIDE
AnnounceDate	2021-05-27
AnnouncementXML	Submission_2021-05-27_07:03:46.957.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022167
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kevin Howe
SpeciesList	scientific name: Myzus persicae; NCBI TaxID: 13164; scientific name: Potato leafroll virus; NCBI TaxID: 12045;
ModificationList	methylthiolated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 01:29:18	ID requested
⏵ 1	2021-05-27 07:03:47	announced

DeBlasio SL, Wilson JR, Tamborindeguy C, Johnson RS, Pinheiro PV, MacCoss MJ, Gray SM, Heck M, Affinity Purification-Mass Spectrometry Identifies a Novel Interaction between a Polerovirus and a Conserved Innate Immunity Aphid Protein that Regulates Transmission Efficiency. J Proteome Res, 20(6):3365-3387(2021) [pubmed]

submitter keyword: Potato leafroll virus, PLRV, Luteoviridae, polerovirus, aphid, circulative transmission, phloem-limited plant virus, Myzus persicae, potato pathogen, AP-MS.

Michelle Heck
contact affiliation	USDA-ARS, Cornell University, Boyce Thompson Institute
contact email	mlc68@cornell.edu
lab head
Kevin Howe
contact affiliation	USDA-ARS
contact email	kjh46@cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022167
     1. Label: PRIDE project
     2. Name: Isolation of aphid-Potato leafroll virus (PLRV) proteins complexes from viruliferous insects using affinity purification-mass spectrometry