PXD022165

PXD022165 is an original dataset announced via ProteomeXchange.

Title	Proteomic insights into senescence of testicular peritubular cells from a nonhuman primate model
Description	Age-related changes of the human testis may include morphological alterations, disturbed steroidogenesis and impaired spermatogenesis. However, the specific impact of cell age remains poorly understood and difficult to assess. Testicular peritubular cells fulfill essential functions, including sperm transport, contributions to the spermatogonial stem cell niche and paracrine interactions within the testis. To study their role in age-associated decline of testicular functions, we performed comprehensive proteome and secretome analyses of repeatedly passaged peritubular cells from Callithrix jacchus. This nonhuman primate model better reflects the human testicular biology than rodents and further gives access to young donors unavailable from humans. Among 5095 identified proteins, 583 were differentially abundant between samples with low and high passage numbers. The alterations indicate a reduced ability of senescent peritubular cells to contract and secrete proteins, as well as disturbances in NF-κB signaling and a reduced capacity to handle reactive oxygen species. Since this in vitro model may not exactly mirror all molecular aspects of in vivo aging, we investigated the proteomes and secretomes of testicular peritubular cells from young and old donors. Even though the age-related alterations at the protein level were less pronounced, we found evidence for impaired protein secretion, altered NF-κB signaling and reduced contractility of these in vivo aged peritubular cells.
HostingRepository	PRIDE
AnnounceDate	2020-11-18
AnnouncementXML	Submission_2020-11-18_02:58:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jan Bernd Stöckl
SpeciesList	scientific name: Callithrix jacchus; NCBI TaxID: 9483;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 01:10:46	ID requested
⏵ 1	2020-11-18 02:58:38	announced

Dataset with its publication pending

submitter keyword: Ageing, testis, cellular model, proteome, marmoset monkey, non-human primate, senescence, secretome

Thomas Fröhlich
contact affiliation	Laboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany
contact email	frohlich@genzentrum.lmu.de
lab head
Jan Bernd Stöckl
contact affiliation	Genzentrum der LMU München
contact email	stoeckl@genzentrum.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD022165

PRIDE project URI

• PRIDE
  1. PXD022165
     1. Label: PRIDE project
     2. Name: Proteomic insights into senescence of testicular peritubular cells from a nonhuman primate model