PXD022163

PXD022163 is an original dataset announced via ProteomeXchange.

Title	Analysing the Mechanism of Mitochondrial Oxidation-induced Cell Death Using a Multifunctional Iridium(III) Photosensitiser
Description	Mitochondrial oxidation-induced cell death, a physiological process triggered by various cancer therapeutics to induce oxidative stress on tumours, has been challenging to investigate owing to the difficulties in generating mitochondria-specific oxidative stress and monitoring mitochondrial responses simultaneously. Accordingly, to the best of our knowledge, the relationship between mitochondrial protein oxidation via oxidative stress and the subsequent cell death-related biological phenomena has not been defined. Here, we developed a multifunctional iridium(III) photosensitiser, Ir-OA, capable of inducing substantial mitochondrial oxidative stress and monitoring the corresponding change in viscosity, polarity, and morphology. Photoactivation of Ir-OA triggers chemical modifications in mitochondrial protein-crosslinking and oxidation (i.e., Oxidative phosphorylation complexes and channel and translocase proteins), leading to microenvironment changes, such as increased microviscosity and depolarisation. These changes are strongly related to cell death by inducing mitochondrial swelling with excessive fission and fusion. We suggest a potential mechanism from mitochondrial oxidative stress to cell death based on proteomic analyses and phenomenological observations.
HostingRepository	PRIDE
AnnounceDate	2021-03-23
AnnouncementXML	Submission_2021-03-23_11:58:59.115.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022163
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tae-Hyuk Kwon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 00:29:10	ID requested
⏵ 1	2021-03-23 11:59:00	announced

Lee C, Nam JS, Lee CG, Park M, Yoo CM, Rhee HW, Seo JK, Kwon TH, Analysing the mechanism of mitochondrial oxidation-induced cell death using a multifunctional iridium(III) photosensitiser. Nat Commun, 12(1):26(2021) [pubmed]

submitter keyword: Mitochondrial oxidation-induced cell death, Photosensitiser, Iridium(III) complex, Depolarisation, Photo-crosslinking

Tae-Hyuk Kwon
contact affiliation	Department of Chemistry and Center for Wave Energy Materials, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea
contact email	kwon90@unist.ac.kr
lab head
Tae-Hyuk Kwon
contact affiliation	Ulsan National Institute of Science and Technology (UNIST)
contact email	kwon90@unist.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022163
     1. Label: PRIDE project
     2. Name: Analysing the Mechanism of Mitochondrial Oxidation-induced Cell Death Using a Multifunctional Iridium(III) Photosensitiser