PXD022161

PXD022161 is an original dataset announced via ProteomeXchange.

Title	Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B-related hepatocellular carcinoma
Description	Lysine acetylation (Kac) as an important post-translational modification of histone is essential for gene expression regulation in hepatocellular carcinoma (HCC), however the atlas of whole acetylated proteins for HCC tissue and the difference of protein acetylation between human normal and HCC tissues are unknown. In this report, we characterized the proteome and acetyl proteome (acetylome) profile of normal, paracancerous and HCC liver tissues in human clinical samples by quantitative proteomics techniques. We identified 6,781 acetylation sites of 2,582 proteins and quantified 2,492 acetylation sites from 1,190 proteins in normal, paracancerous and HCC liver tissues. Among them fifteen proteins were hyperacetylated with more than ten lysine residues. And the histone acetyltransferases p300 and CBP were found to be hyperacetylated in hepatitis B virus pathway. Moreover, we found that 250 Kac sites of 214 proteins were up-regulated and 662 Kac site of 451 proteins were down-regulated in HCC compared with normal liver tissues. And the acetylation levels of lysine 120 in histone H2B (H2BK120ac), lysine 18 in histone H3.3 (H3.3K18ac) and lysine 77 in histone H4 (H4K77ac) were increased in HCC. More interestingly, the H2BK120ac and H3.3K18ac significantly associated with survival, and H4K77ac highly associated with survival and recurrence in an independent clinical cohort of HCC patients. Overall, this study lays a foundation for understanding the functions of acetylation in hepatocellular carcinoma and provides potential prognostic factors for the diagnosis and therapy of HCC.
HostingRepository	iProX
AnnounceDate	2020-10-24
AnnouncementXML	Submission_2022-11-22_00:39:47.970.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoqiang Chai
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-25 08:33:40	ID requested
⏵ 1	2022-11-22 00:39:48	announced

Chai X, Guo J, Dong R, Yang X, Deng C, Wei C, Xu J, Han W, Lu J, Gao C, Gao D, Huang C, Ke A, Li S, Li H, Tian Y, Gu Z, Liu S, Liu H, Chen Q, Liu F, Zhou J, Fan J, Shi G, Wu F, Cai J, Quantitative acetylome analysis reveals histone modifications that may predict prognosis in hepatitis B-related hepatocellular carcinoma. Clin Transl Med, 11(3):e313(2021) [pubmed]

submitter keyword: hepatocellular carcinoma, proteomics, histone, lysine acetylation, prognostic factor

Jiabin Cai
contact affiliation	Department of Liver Surgery and Transplantation of Zhongshan Hospital, Liver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200032, China
contact email	cai.jiabin@zs-hospital.sh.cn
lab head
Xiaoqiang Chai
contact affiliation	Department of Liver Surgery and Transplantation of Zhongshan Hospital, Liver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200032, China
contact email	18111510037@fudan.edu.cn
dataset submitter

iProX dataset URI