PXD022156

PXD022156 is an original dataset announced via ProteomeXchange.

Title	Development of a mass-spectrometry based method for the identification of the in vivo whole blood and plasma ADP-ribosylomes
Description	Blood and plasma proteins are heavily investigated as biomarkers for different diseases. However, the post-translational modifications of these proteins are rarely analyzed since blood contains many enzymes that rapidly remove the modification after sampling. In contrast to the well-described role of protein ADP-ribosylation in cells and organs, its role in blood remains mostly uncharacterized. Here, we established that plasma phosphodiesterases and ADP-ribosylhydrolases rapidly demodified in vitro ADP-ribosylated proteins. Thus, to identify the in vivo whole blood and plasma ADP-ribosylomes, we established a novel mass-spectrometry based workflow that was applied to blood samples collected from LPS-treated pigs (Sus scrofa), which serves as a model for human systemic inflammatory response syndrome. These analyses identified 60 ADP-ribosylated proteins, 17 of which were ADP-ribosylated plasma proteins. This new protocol provides an important step forward for the rapidly developing field of ADP-ribosylation and defines the blood and plasma ADP-ribosylomes under both healthy and disease conditions.
HostingRepository	PRIDE
AnnounceDate	2021-05-28
AnnouncementXML	Submission_2021-05-28_06:29:48.757.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Deena Leslie Pedrioli
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-23 06:50:40	ID requested
⏵ 1	2021-05-28 06:29:49	announced

L, ü, thi SC, Howald A, Nowak K, Graage R, Bartolomei G, Neupert C, Sidler X, Leslie Pedrioli D, Hottiger MO, Whole Blood and Plasma ADP-Ribosylomes. J Proteome Res, 20(6):3090-3101(2021) [pubmed]

submitter keyword: ADP-ribosylation, systemic inflammatory response syndrome, sepsis, blood, plasma, histidine ADP-ribosylation

Michael O. Hottiger, Prof. PhD, DVM
contact affiliation	Head of Department Department of Molecular Mechanisms of Disease University of Zurich Winterthurerstr. 190 8057 Zurich, Switzerland
contact email	michael.hottiger@dmmd.uzh.ch
lab head
Deena Leslie Pedrioli
contact affiliation	University of Zürich
contact email	deena.lesliepedrioli@dmmd.uzh.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022156
     1. Label: PRIDE project
     2. Name: Development of a mass-spectrometry based method for the identification of the in vivo whole blood and plasma ADP-ribosylomes