PXD022150

PXD022150 is an original dataset announced via ProteomeXchange.

Title	Identification of microbial-derived HLA-bound peptides in melanoma
Description	Various bacterial species are known to colonize human tumors , proliferate within them and modulate immune function, ultimately affecting patient survival and response to treatment. However, it is not known whether intracellular bacterial antigens are presented by HLA-I and HLA-II molecules of tumor cells, and whether potential tumor-presented bacterial-derived antigens may elicit a tumor infiltrating T-cell immune response. Here, we used 16S rRNA gene sequencing and HLA-peptidomics to unbiasedly identify an intracellular bacterial peptide repertoire presented on HLA-I and HLA-II molecules in melanoma tumors. Our microbial analysis of 17 melanoma metastases, derived from 9 patients, revealed 248 and 38 unique HLA-I and HLA-II peptides, respectively, derived from 41 different classified bacterial species. Recurrent bacterial peptides were identified in different tumors. Our study reveals that intra-tumor intracellular bacteria can be presented by tumor cells and elicit immune-reactivity, thus providing insight into how bacteria influence immune system activation and response to therapy.
HostingRepository	PRIDE
AnnounceDate	2021-02-04
AnnouncementXML	Submission_2021-03-21_22:48:39.868.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shelly Kalaora
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-23 01:25:36	ID requested
1	2021-02-04 04:40:43	announced
⏵ 2	2021-03-21 22:48:40	announced	2021-03-22: Updated publication reference for PubMed record(s): 33731925.

Kalaora S, Nagler A, Nejman D, Alon M, Barbolin C, Barnea E, Ketelaars SLC, Cheng K, Vervier K, Shental N, Bussi Y, Rotkopf R, Levy R, Benedek G, Trabish S, Dadosh T, Levin-Zaidman S, Geller LT, Wang K, Greenberg P, Yagel G, Peri A, Fuks G, Bhardwaj N, Reuben A, Hermida L, Johnson SB, Galloway-Pe, ñ, a JR, Shropshire WC, Bernatchez C, Haymaker C, Arora R, Roitman L, Eilam R, Weinberger A, Lotan-Pompan M, Lotem M, Admon A, Levin Y, Lawley TD, Adams DJ, Levesque MP, Besser MJ, Schachter J, Golani O, Segal E, Geva-Zatorsky N, Ruppin E, Kvistborg P, Peterson SN, Wargo JA, Straussman R, Samuels Y, Identification of bacteria-derived HLA-bound peptides in melanoma. Nature, 592(7852):138-143(2021) [pubmed]

submitter keyword: Human, HLA peptidomics, immunopeptidomics, microbiome, LC-MSMS

Prof. Yardena Samuels
contact affiliation	Weizmann Institute of Science Israel
contact email	Yardena.Samuels@weizmann.ac.il
lab head
Shelly Kalaora
contact affiliation	Weizmann institute of science
contact email	shelly.kalaora@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022150

PRIDE project URI

• PRIDE
  1. PXD022150
     1. Label: PRIDE project
     2. Name: Identification of microbial-derived HLA-bound peptides in melanoma