PXD022146

PXD022146 is an original dataset announced via ProteomeXchange.

Title	MigExpress discovers essential cell migration control genes in non-small cell lung cancer
Description	Cell migration is an essential process in health and disease - especially in cancer metastasis. The difficulty to assess migration in high throughput presents a methodological hurdle - hence, only very few screens revealed factors controlling this important process. Here, we introduce MigExpress as a platform for the "identification of Migration control genes by differential Expression". MigExpress exploits the combination of in-depth molecular profiling and the robust quantitative analysis of migration capacity in a broad panel of samples and identifies migration-associated genes by their differential expression in slowly versus fast migrating cells. We applied MigExpress to non-small cell lung cancer (NSCLC), the most frequent cause of cancer mortality mainly due to metastasis. In 54 NSCLC cell lines, we comprehensively determined mRNA and protein expression. Correlating the transcriptome and proteome profiles with the quantified migration properties led to the discovery and validation of FLNC, DSE, CPA4, TUBB6 and BICC1 as migration control factors in NSCLC cells, which also negatively correlated with patient survival. Notably, FLNC was the least expressed filamin in NSCLC, but the only one controlling cell migration and correlating with patient survival and metastatic disease stage.
HostingRepository	PRIDE
AnnounceDate	2021-04-20
AnnouncementXML	Submission_2021-04-20_09:24:00.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chen Meng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-22 23:38:35	ID requested
⏵ 1	2021-04-20 09:24:00	announced

Dataset with its publication pending

submitter keyword: Cell migration, non-small cell lung cancer (NSCLC), proteomics, transcriptomics, Tandem mass tags (TMT), isobaric labeling

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University of Munich (TUM), Freising, Germany
contact email	tina.ludwig@tum.de
lab head
Chen Meng
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry
contact email	chen.meng@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD022146

PRIDE project URI

• PRIDE
  1. PXD022146
     1. Label: PRIDE project
     2. Name: MigExpress discovers essential cell migration control genes in non-small cell lung cancer