PXD022142

PXD022142 is an original dataset announced via ProteomeXchange.

Title	Female mating status influences protein composition of mature Drosophila oocytes
Description	Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. We investigated whether mating influences mature oocyte composition in Drosophila melanogaster using a quantitative proteomic approach. Our analyses robustly identified 4,485 oocyte proteins and revealed that stage-14 oocytes from mated females differed significantly in protein composition relative to oocytes from unmated females. Proteins forming a highly interconnected network enriched for translational machinery and transmembrane proteins were increased in oocytes from mated females, including calcium binding and transport proteins. This mating-induced modulation of oocyte maturation was also significantly associated with proteome changes that are known to be triggered by egg activation. We propose that these compositional changes are likely to have fitness consequences and adaptive implications given the importance of oocyte composition, rather than active gene expression, to the maternal-to-zygotic transition and early embryogenesis.
HostingRepository	PRIDE
AnnounceDate	2021-02-25
AnnouncementXML	Submission_2021-02-24_22:00:41.822.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Caitlin McDonough-Goldstein
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-22 23:07:26	ID requested
1	2021-02-24 08:18:15	announced
⏵ 2	2021-02-24 22:00:42	announced	2021-02-25: Updated project metadata.

10.1038/S41598-021-82801-4;

McDonough-Goldstein CE, Pitnick S, Dorus S, Drosophila oocyte proteome composition covaries with female mating status. Sci Rep, 11(1):3142(2021) [pubmed]

submitter keyword: oogenesis, oocyte, parental investment, maternal effects, Drosophila, LC-MSMS, TMT

Steve Dorus
contact affiliation	Department of Biology, Syracuse University, Syracuse, NY, USA
contact email	sdorus@syr.edu
lab head
Caitlin McDonough-Goldstein
contact affiliation	Syracuse University
contact email	mcdonouce@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022142

PRIDE project URI

• PRIDE
  1. PXD022142
     1. Label: PRIDE project
     2. Name: Female mating status influences protein composition of mature Drosophila oocytes