PXD022133

PXD022133 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of PMJ2-R and primary peritoneal macrophages
Description	In vitro models are often used to study the functions of macrophages, including the process of phagocytosis. The use of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization of the results obtained. This disadvantage is lacking in immortalized cell lines. The PMJ2-R cell line was derived from in vivo immortalization and retains many of the primary peritoneal macrophages functions, but little is known about its differences from normal cells. In this article, we carried out a comparative analysis of the proteomes of PMJ2-R cells and primary peritoneal macrophages isolated from the C57BL/J6 mice. Particular attention was paid to the analysis of proteins involved in the process of phagocytosis. A total of 4005 proteins were identified, of which 797 were quantified. Our results indicate that there are significant differences in the abundance of a large number of proteins, including important proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, Msr1. Thus, when using PMJ2R cells as model cells in the study of the peritoneal macrophages functions, features revealed in this study should be taken into account.
HostingRepository	PRIDE
AnnounceDate	2021-07-12
AnnouncementXML	Submission_2021-07-11_22:19:17.531.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olga Tikhonova
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-22 05:47:35	ID requested
⏵ 1	2021-07-11 22:19:18	announced

Rusanov AL, Kozhin PM, Tikhonova OV, Zgoda VG, Loginov DS, Chlast, á, kov, á A, Selinger M, Sterba J, Grubhoffer L, Luzgina NG, Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages. Int J Mol Sci, 22(12):(2021) [pubmed]

submitter keyword: PMJ2-R, peritoneal macrophages, phagocytosis, proteome, LC-MS/MS

Natalia G. Luzgina
contact affiliation	The Precision Biosystems Laboratory, Institute of Biomedical Chemistry
contact email	ngluzgina@gmail.com
lab head
Olga Tikhonova
contact affiliation	Institute of Biomedical Chemistry
contact email	ovt.facility@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD022133

PRIDE project URI

• PRIDE
  1. PXD022133
     1. Label: PRIDE project
     2. Name: Proteome profiling of PMJ2-R and primary peritoneal macrophages