PXD022132

PXD022132 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Glycoproteomic analysis of fucose-containing proteins in small intestine identified cryptdin-related sequence 1 as O-glycosylated proteins modified with α1,2-fucose
Description	The modification of galactose with α1,2-fucose is involved in symbiosis with intestinal bacteria and elimination of pathogenic bacteria. It is postulated that α1,2-fucosylated mucin secreted from goblet cells is involved in defending an organism against infections, but the detailed molecular mechanisms are yet to be elucidated. It was previously reported that Paneth cells of the small intestine were positive for UEA-1 lectin staining. However, glycoproteins in Paneth cells carrying α1,2-fucose have not yet been identified. Glycoproteomic analysis of ileal lysates identified 3212 O-linked and 2962 N-linked glycopeptides. In particular, cryptdin-related sequence 1 (CRS1) expressed in Paneth cells was found to be α1,2-fucosylated. Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform. Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
HostingRepository	jPOST
AnnounceDate	2020-11-05
AnnouncementXML	Submission_2022-09-18_02:54:09.900.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hiroki Hashiguchi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-22 02:04:20	ID requested
1	2020-11-05 01:44:59	announced
2	2020-11-10 08:10:52	announced	2020-11-11: Updated PubMed.
3	2021-02-04 17:14:44	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 02:54:10	announced	2022-09-18: Updated FTP location.

Publication List

Hashiguchi H, Tsukamoto Y, Ogawa M, Tashima Y, Takeuchi H, Nakamura M, Kawashima H, Fujishiro M, Okajima T, 1,2-fucose in the small intestine. Arch Biochem Biophys, 695():108653(2020) [pubmed]

Hashiguchi H, Tsukamoto Y, Ogawa M, Tashima Y, Takeuchi H, Nakamura M, Kawashima H, Fujishiro M, Okajima T, 1,2-fucose in the small intestine. Arch Biochem Biophys, 695():108653(2020) [pubmed]

Keyword List

submitter keyword: mouse, glycopeptide, glycan, TMT, CRS1, defensin

submitter keyword: mouse, glycopeptide, glycan, TMT, CRS1, defensin

Contact List

Tetsuya Okajima
lab head
Hiroki Hashiguchi
contact affiliation	Nagoya University Graduate School of Medicine
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000988/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000988/