PXD022107

PXD022107 is an original dataset announced via ProteomeXchange.

Title	The hepatovirus pX protein contains ancient signals for ESCRT-dependent capsid quasi-envelopment
Description	Hepatoviruses, a common cause of acute hepatitis in humans, are atypical picornaviruses released from cells in small vesicles resembling exosomes. We show the nonlytic release of these quasi-enveloped virions is mediated by a C-terminal extension of the VP1 capsid protein (pX) that binds the Bro1 domains of ALIX and HD-PTP, and recruits the ubiquitin ligase ITCH to drive an association with endosomal sorting complexes required for transport (ESCRT). Fusing pX to a self-assembling, de novo designed nanocage protein resulted in ESCRT-dependent release mediated by a 20-amino acid core sequence containing a Y[KR]xxR[LM] motif conserved in viruses from bats to humans. Mutations in this motif ablate release and lead virus to accumulate intracellularly. Our study identifies an exceptionally potent viral export signal mediating extracellular release of virus-sized protein assemblies, identifies its key cellular binding partners, and shows non-lytic release of quasi-enveloped virus is a tightly orchestrated and ancient evolutionary trait of hepatoviruses.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:38:30.905.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ling Xie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-21 02:33:27	ID requested
1	2022-08-02 07:44:01	announced
2	2023-11-14 08:51:24	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:38:31	announced	2024-10-22: Updated project metadata.

10.1371/JOURNAL.PPAT.1010543;

submitter keyword: hepatovirus pX, LC-MS

Stanley M. Lemon
contact affiliation	Department of Medicine, Department of Microbiology & Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
contact email	smlemon@med.unc.edu
lab head
Ling Xie
contact affiliation	UNC at Chapel Hill
contact email	xiel@email.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD022107

PRIDE project URI

• PRIDE
  1. PXD022107
     1. Label: PRIDE project
     2. Name: The hepatovirus pX protein contains ancient signals for ESCRT-dependent capsid quasi-envelopment