PXD022104

PXD022104 is an original dataset announced via ProteomeXchange.

Title	The ER-embedded UBE2J1/RNF26 ubiquitylation complex in spatio-temporal control of the endolysosomal pathwayThe ER-embedded UBE2J1/RNF26 ubiquitylation complex in spatio-temporal control of the endolysosomal pathway
Description	The endolysosomal system fulfills a wide variety of cellular functions, many of which are modulated through interactions with other organelles. In particular, the ER exerts spatiotemporal constraints on the organization and motility of endosomes and lysosomes. We have recently described the ER transmembrane E3 ubiquitin ligase RNF26 as a regulator of perinuclear positioning and transport dynamics of the endolysosomal network. We now report that the ubiquitin conjugating enzyme UBE2J1, also anchored in the ER membrane, collaborates with RNF26 in this context, and that the cellular activity of this E2/E3 pair, localized in a perinuclear ER subdomain, is supported by transmembrane interactions. Through modification of SQSTM1/p62 on lysine 435, the ER-embedded UBE2J1/RNF26 ubiquitylation complex recruits endosomal adaptors to immobilize their cognate vesicles in the perinuclear region of the cell. The resulting spatiotemporal compartmentalization of the endolysosomal system promotes trafficking of activated EGFR to the late compartments and facilitates cessation of downstream AKT signaling.
HostingRepository	PRIDE
AnnounceDate	2021-01-22
AnnouncementXML	Submission_2021-01-21_23:49:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fredrik Trulsson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine; phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-21 02:33:15	ID requested
⏵ 1	2021-01-21 23:49:17	announced

Cremer T, Jongsma MLM, Trulsson F, Vertegaal ACO, Neefjes J, Berlin I, The ER-embedded UBE2J1/RNF26 ubiquitylation complex exerts spatiotemporal control over the endolysosomal pathway. Cell Rep, 34(3):108659(2021) [pubmed]

submitter keyword: UBE2J1, RNF26, endoplasmic reticulum, endosomes, ubiquitin, EGFR

Alfred Vertegaal
contact affiliation	Department of Cell and Chemical Biology at the Leiden University Medical Center.
contact email	Vertegaal@lumc.nl
lab head
Fredrik Trulsson
contact affiliation	LUMC
contact email	c.f.b.trulsson@lumc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022104
     1. Label: PRIDE project
     2. Name: The ER-embedded UBE2J1/RNF26 ubiquitylation complex in spatio-temporal control of the endolysosomal pathwayThe ER-embedded UBE2J1/RNF26 ubiquitylation complex in spatio-temporal control of the endolysosomal pathway