PXD022098 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Translation Error Clusters Induced by Aminoglycoside Antibiotics |
Description | Aminoglycoside antibiotics target the ribosome and induce mistranslation, yet which translation errors induce bacterial cell death is unclear. The analysis of cellular proteins by quantitative mass spectrometry shows that bactericidal aminoglycosides induce not only single errors, but also clusters of errors in full-length proteins in vivo with as much as four amino acid substitutions in a row. The downstream errorsin a cluster are up to 10,000-fold more frequent than the first error and independent of the intracellular aminoglycoside concentration. The prevalence, length, and composition of error clusters dependsnot only on the misreading propensity of a given aminoglycoside, but also on its ability to inhibit ribosome translocation along the mRNA. Error clusters constitute a new class of misreading events in vivo that may provide the predominant source of proteotoxic stress at low aminoglycoside concentration, which is particularly important for the autocatalytic uptake of the drugs. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-06 |
AnnouncementXML | Submission_2021-04-06_00:25:53.481.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ingo Wohlgemuth |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-20 07:32:57 | ID requested | |
⏵ 1 | 2021-04-06 00:25:54 | announced | |
Publication List
Wohlgemuth I, Garofalo R, Samatova E, G, ü, nen, ç AN, Lenz C, Urlaub H, Rodnina MV, Translation error clusters induced by aminoglycoside antibiotics. Nat Commun, 12(1):1830(2021) [pubmed] |
Keyword List
submitter keyword: aminoglycosides |
Contact List
Ingo Wohlgemuth |
contact affiliation | Max Planck Institute for Biophysical Chemistry Department of Physical Biochemistry Am Fassberg 11 37077 Goettingen Germany |
contact email | Ingo.Wohlgemuth@mpibpc.mpg.de |
lab head | |
Ingo Wohlgemuth |
contact affiliation | Max-Planck Institute for Biophysical chemistry |
contact email | Ingo.Wohlgemuth@mpibpc.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022098
- Label: PRIDE project
- Name: Translation Error Clusters Induced by Aminoglycoside Antibiotics