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PXD022098

PXD022098 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTranslation Error Clusters Induced by Aminoglycoside Antibiotics
DescriptionAminoglycoside antibiotics target the ribosome and induce mistranslation, yet which translation errors induce bacterial cell death is unclear. The analysis of cellular proteins by quantitative mass spectrometry shows that bactericidal aminoglycosides induce not only single errors, but also clusters of errors in full-length proteins in vivo with as much as four amino acid substitutions in a row. The downstream errorsin a cluster are up to 10,000-fold more frequent than the first error and independent of the intracellular aminoglycoside concentration. The prevalence, length, and composition of error clusters dependsnot only on the misreading propensity of a given aminoglycoside, but also on its ability to inhibit ribosome translocation along the mRNA. Error clusters constitute a new class of misreading events in vivo that may provide the predominant source of proteotoxic stress at low aminoglycoside concentration, which is particularly important for the autocatalytic uptake of the drugs.
HostingRepositoryPRIDE
AnnounceDate2021-04-06
AnnouncementXMLSubmission_2021-04-06_00:25:53.481.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIngo Wohlgemuth
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-20 07:32:57ID requested
12021-04-06 00:25:54announced
Publication List
Wohlgemuth I, Garofalo R, Samatova E, G, ü, nen, ç AN, Lenz C, Urlaub H, Rodnina MV, Translation error clusters induced by aminoglycoside antibiotics. Nat Commun, 12(1):1830(2021) [pubmed]
Keyword List
submitter keyword: aminoglycosides
Contact List
Ingo Wohlgemuth
contact affiliationMax Planck Institute for Biophysical Chemistry Department of Physical Biochemistry Am Fassberg 11 37077 Goettingen Germany
contact emailIngo.Wohlgemuth@mpibpc.mpg.de
lab head
Ingo Wohlgemuth
contact affiliationMax-Planck Institute for Biophysical chemistry
contact emailIngo.Wohlgemuth@mpibpc.mpg.de
dataset submitter
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