PXD022097

PXD022097 is an original dataset announced via ProteomeXchange.

Title	Rat motor and sensory nerve LC-MSMS
Description	Peripheral nerve repair and functional recovery depend on the rate of nerve regeneration and the quality of target reinnervation. It is important to fully understand the cellular and molecular basis underlying the specificity of peripheral nerve regeneration, which means the achieving of respective correct pathfinding and accurate target reinnervation for regrowing motor and sensory axons. In this study, a quantitative proteomic technique, based on isobaric tags for relative and absolute quantitation (iTRAQ) was used to profile the protein expression pattern between single motor and sensory nerves at 14 days after peripheral nerve transection. Among a total of 1259 proteins identified, 176 proteins showed the differential expressions between injured motor and sensory nerves. Quantitative real-time RT-PCR and Western blot analysis were applied to validate the proteomic data on representative differentially expressed proteins. Functional categorization indicated that differentially expressed proteins were linked to a diverse array of molecular functions, including axonogenesis, response to axon injury, tissue remodeling, axon ensheathment, cell proliferation and adhesion, vesicle-mediated transport, response to oxidative stress, internal signal cascade, and macromolecular complex assembly, which might play an essential role in peripheral motor and sensory nerve regeneration. Overall, we hope that the proteomic database obtained in this study could serve as a solid foundation for the comprehensive investigation of differentially expressed proteins between injured motor and sensory nerves and for the mechanism elucidation of the specificity of peripheral nerve regeneration.
HostingRepository	PRIDE
AnnounceDate	2020-12-10
AnnouncementXML	Submission_2020-12-10_01:25:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhua JI
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-20 07:19:38	ID requested
⏵ 1	2020-12-10 01:25:39	announced

He Q, Yu F, Cong M, Ji Y, Zhang Q, Ding F, Comparative Proteomic Analysis of Differentially Expressed Proteins between Injured Sensory and Motor Nerves after Peripheral Nerve Transection. J Proteome Res, 20(3):1488-1508(2021) [pubmed]

submitter keyword: Rat, Motor nerve, Sensory nerve, Peripheral nerve injury, LC-MSMS

Yuhua Ji
contact affiliation	Key Laboratory of Neuroregeneration, Nantong University, Nantong, China.
contact email	tjiyuhua@126.com
lab head
Yuhua JI
contact affiliation	Department of Immunobiology, College of Life Science and Technology, Jinan University, Guangzhou, China.
contact email	tjiyuhua@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD022097

PRIDE project URI

• PRIDE
  1. PXD022097
     1. Label: PRIDE project
     2. Name: Rat motor and sensory nerve LC-MSMS