PXD022095

PXD022095 is an original dataset announced via ProteomeXchange.

Title	CD49f defines a subset of dysfunctional regulatory T cells circulating in the human peripheral blood
Description	The therapeutic use of regulatory T cells (Tregs) in patients with autoimmune disorders has been hampered by the biological variability of memory Treg populations in the peripheral blood. In this study, we reveal through a combination of quantitative proteomic, multiparametric flow cytometry, RNA-seq data analysis and functional assays, that CD49f is heterogeneously expressed among human Tregs and impacts their immunomodulatory function. High expression of CD49f defines a subset of dysfunctional Tregs in the human blood characterized by a Th17-like phenotype and impaired suppressive capacity. CD49f is similarly distributed between naïve and memory Tregs and impacts the expression of CD39, CTLA-4, FoxP3 and CCR6 specifically in the memory compartment. Accumulation of CD49f high memory Tregs in the blood of ulcerative colitis patients correlates with disease severity. Our results highlight important considerations for Treg immunotherapy design in patients with inflammatory bowel disease which could possibly extend to other autoimmune disorders.
HostingRepository	PRIDE
AnnounceDate	2021-08-06
AnnouncementXML	Submission_2022-01-11_02:55:55.794.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harshi Weerakoon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-20 07:19:30	ID requested
1	2021-08-06 02:34:49	announced
⏵ 2	2022-01-11 02:55:56	announced	2022-01-11: Updated publication reference for PubMed record(s): 34950757.

Weerakoon H, Miles JJ, Lepletier A, Hill MM, A high-resolution mass spectrometry based proteomic dataset of human regulatory T cells. Data Brief, 40():107687(2022) [pubmed]

submitter keyword: Human, Regulatory T cells, Shotgun proteomics

Michelle M. Hill
contact affiliation	Precision and Systems Biomedicine Laboratory, QIMR Berghofer Medical Research Institute, Herston, QLD, Australia
contact email	michelle.hill@qimrberghofer.edu.au
lab head
Harshi Weerakoon
contact affiliation	Senior lecturer, Department of Biochemistry, Faculty of Medicine and Allied Sciences, Rajarata University of Sri Lanka, Saliyapura, Sri Lanka Visiting Scientists, Precision and Systems Biomedicine group, QIMR Berghofer Medical Reserach Institute, Brisbane, Australia
contact email	harshitw83@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD022095

PRIDE project URI

• PRIDE
  1. PXD022095
     1. Label: PRIDE project
     2. Name: CD49f defines a subset of dysfunctional regulatory T cells circulating in the human peripheral blood