PXD022090

PXD022090 is an original dataset announced via ProteomeXchange.

Title	the raw MS/MS data(Piglets testis LC-MS/MS analysis)
Description	1α,25(OH)2VD3 is the most active form of VD3 in animals and it plays an important role in regulating mineral metabolism but also reproduction and immunity. Testes are the main reproductive organs of male mammals. In order to study the effect of 1α,25(OH)2VD3 on early testicular development, 140 weaned 21-day old piglets were selected. The piglets were randomly divided into four groups and were fed a commercial diet supplemented with 0, 1, 2 and 4 μg.kg-1 of 1α,25(OH)2VD3, provided as 1α,25(OH)2VD3-glycosides, 60 days after the start of the experiment, at piglet age 82 days, testes were harvested. The morphology and histology of early testicular development were assessed. In addition, the proteomic TMT/iTRAQ labeling technique was used to analyze the protein profile of the testes in each group. Western blotting was used to verify the target of differentially abundant proteins (DAPs). The results showed that of the identified 132715 peptides, 122755 were specific peptides. 7852 proteins, of which 6573 proteins contain quantitative information. Screening for DAPs was focused on proteins closely related to the regulation of the testicular development such as steroid hormone synthesis, steroid biosynthesis, peroxisome and fatty acid metabolism pathways. These results also indicate that 1α,25(OH)2VD3 is involved in the regulation of early testicular development in piglets. At the same time, these findings provide valuable information for the proteins involved in the regulation of testicular development, and help to better understand the mechanisms of 1α,25(OH)2VD3 in regulating the development of piglets’ testes. Significance: The early development of the testes of breeding boars is closely related to their lifetime fertility. Our research identifies potential signaling pathways and differentially abundant proteins associated with testicular development. These findings indicate that plants containing 1α,25(OH)2VD3-glycosides used as a nutritional ingredient may help early testicular development in breeding boars and reveal the regulatory mechanism of 1α,25(OH)2VD3 on testicular development.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_10:33:44.806.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	wanghong liu
SpeciesList	scientific name: Sus scrofa scrofa; NCBI TaxID: 415978;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-20 01:15:22	ID requested
⏵ 1	2021-09-09 10:33:45	announced

Chen H, B, ü, hler K, Zhu Y, Nie X, Liu W, -glycosides on development of early testes in piglets. Sci Rep, 11(1):11341(2021) [pubmed]

submitter keyword: 1α,25(OH)2VD3-glycosides;Piglet testes development;Proteomics;TMT/iTRAQ Labeling;Differentially abundant proteins

Wanghong Liu
contact affiliation	College of Animal Science and Technology, Huazhong Agricultural University, No.1 Shizishan Road, Hongshan District, Wuhan 430070, China National Engineering and Technology Research Center For Livestock; The Breeding Swine Quality Supervision and Testing Center, Ministry of Agriculture, Wuhan 430070, China
contact email	whliu@mail.hzau.edu.cn
lab head
wanghong liu
contact affiliation	College of Animal Science and Technology, Huazhong Agricultural University, No.1 Shizishan Road, Hongshan District, Wuhan 430070, China National Engineering and Technology Research Center For Livestock; The Breeding Swine Quality Supervision and Testing Center, Ministry of Agriculture, Wuhan 430070, China
contact email	whliu@mail.hzau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022090
     1. Label: PRIDE project
     2. Name: the raw MS/MS data(Piglets testis LC-MS/MS analysis)